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Microbial agent for treating acetone-containing medical and chemical industrial waste water and application thereof

A technology of microorganisms and bacterial agents, applied in the field of microbial agents, can solve problems affecting food safety of agricultural products, ecological system hazards, biological toxicity, etc., and achieve the effects of solving the problem of acetone exceeding the standard, protecting the ecological environment, and reducing production and use costs

Inactive Publication Date: 2013-04-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Acetone, which is common in industrial wastewater, is a toxic and harmful chemical substance. After entering the ecological environment, it not only poisons various organisms, but also causes serious harm to the ecological system.
Organic wastewater containing acetone will cause pollution to the natural water environment, seriously affecting people's health and food safety such as agricultural products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A pharmaceutical and chemical factory in Shangyu City, Zhejiang Province has long-term produced acetone-containing chemical wastewater gradient dilution, and spread it on a 1 / 10LB solid medium plate (10 grams of peptone, 5 grams of yeast extract, 10 grams of NaCl, 20 grams of agar powder, water 1000 ml, pH 7.0-7.2), cultivated in a 30°C incubator for 48 hours, and singled out bacteria for acetone degradation verification.

[0027] After expanding the cultivation of the single bacterium picked, inoculate the inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams of NaCl, 0.5 grams of NaCl, 1000 milliliters of water, pH 7.0~7.2) for degradation verification, the effective single bacteria that have a degradation effect on acetone, and 2 effective single bacteria were obtained. One of the strains was Pseudomonas XHC.

[0028] XHC (CGMCC NO.5106) was identified as Pseudomonas sp. by 16SrDNA. The main biological character...

Embodiment 2

[0031] Determination of degradation characteristics of degrading bacteria XHC (CGMCC NO.5106) in inorganic salt liquid medium: pick XHC single colony in 5ml LB liquid medium (10 grams of peptone, 5 grams of yeast extract, 10 grams of NaCl, 1000 ml of water, pH 7.0 ~7.2), 30°C, 170rmp / min shaking culture for 24h, to obtain XHC (CGMCC NO.5106) fresh bacterial liquid.

[0032] In 100ml of 50mg / L acetone inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams, 0.5 grams of NaCl, 1000 milliliters of water, pH 7.0 ~ 7.2), insert 5 ml of the bacterial liquid after centrifugation and suspension, cultivate in a shaking table at 30°C (170rmp / min), take samples regularly for determination, and measure the content of acetone. See Table 1, the degradation rate of acetone by the degrading bacteria XHC in the inorganic salt liquid medium reaches 75.4%.

[0033] Degradation bacteria XHC (CGMCC NO.5106) in waste water is used to measure the ...

Embodiment 3

[0037] The original seed of the acetone-degrading bacteria XHC (CGMCC NO.5106) of the present invention is activated on LB medium, and inoculated on the slant of the test tube for later use. Slant seed medium formula: 10 grams of peptone, 5 grams of yeast extract, 10 grams of NaCl, 1000 ml of water, 20 grams of agar, pH 7.0-7.2.

[0038] The slant bacteria were inoculated in a 1000ml shaker flask containing 200ml of broth medium (3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 1000 ml of water, pH 7.0 to 7.2), cultured at a constant temperature until the logarithmic phase, ready for inoculation seed jar. 500 liters of seed tank, 400 liters of feeding capacity, medium formula (mass percentage): glucose 0.8%, NH 4 NO 3 1%, K 2 HPO 4 0.2%, MgSO 40.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, the balance is water, pH value 7.2-7.5. After the feeding is completed, 121 ° C high-pressure damp heat sterilization, after cooling to 33 ° C, the above-mentio...

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Abstract

The invention relates to a microbial agent for treating acetone-containing medical and chemical industrial waste water and an application thereof, which belong to the field of environmental-friendly microbes. Pseudomonas sp. XHC is preserved in China General Microbiological Center Culture Collection Center on August 1, 2011, and the strain preservation number is CGMCC No.5106. The pseudomonas XHC(CGMCC No.5106) can degrade more than 70 percent of acetone in medical and chemical industrial waste water and is suitable for being used in factories producing the acetone-containing medical and chemical industrial waste water.

Description

technical field [0001] The invention belongs to the field of environment-friendly microorganisms, and relates to a microbial agent for treating acetone-containing pharmaceutical and chemical wastewater and its application. Background technique [0002] Industrial wastewater such as dyes, coking, and pharmaceuticals all contain a large amount of refractory organic matter. The salient features of this type of wastewater are high concentration, not easy to biodegrade, and also have carcinogenic, teratogenic, and mutagenic properties, which are extremely harmful to the environment and human health. Pollution and poisoning incidents caused by refractory toxic and harmful organic substances are not uncommon, and its treatment has reached the point of no delay. Its research is a research hotspot in the field of water pollution control in the world. [0003] Acetone, which is common in industrial wastewater, is a toxic and harmful chemical substance. After entering the ecological e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14A62D3/02C02F3/34C12R1/38A62D101/28C02F101/34
Inventor 李顺鹏刘亚龙吴志国黄星朱建春
Owner NANJING AGRICULTURAL UNIVERSITY
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