Preparation method and application of atalantia buxjfolia extract
An extract, Dongfeng technology, applied in the field of medicine, can solve the problems such as unclear action mechanism and efficacy of Dongfeng orange, difficult quality control, etc., and achieve the effects of suitable promotion and application, low production cost and simple preparation method.
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Embodiment 1
[0023] The preparation of embodiment 1 Dongfeng orange extract
[0024] (1) Take Dongfeng orange medicinal material (the dry root and stem of Severinia buxifolia (Poir).Oliv.) are used in this embodiment), pulverize, take the coarse powder, and use a volume ratio concentration of 95% ethanol for reflux extraction, For the first time, 5 times the mass of the medicinal material was used for reflux extraction with ethanol, and for the second time, 3 times the mass of the medicinal material was used for reflux extraction, and each reflux extraction was 2 hours, and the second extraction was followed by standing and filtering;
[0025] (2) Merge the filtrate gained in step (1), and reclaim the solvent under conventional reduced pressure to obtain the total extract;
[0026] (3) Suspend the total extract obtained in step (2) with water (6 times the quality of the total extract), then use sherwood oil, chloroform, ethyl acetate and n-butanol (volume of extraction solvent: volume of w...
Embodiment 2
[0029] Application experiment of embodiment 2 Dongfeng orange extract
[0030] (1) Antitussive effect experiment
[0031] 1 Experimental materials
[0032] 1.1 Drugs
[0033] The Dongfeng orange extract prepared in Example 1 of the present invention, including petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract and water extract, were dispersed with 0.5% normal saline respectively to prepare Test solutions with different concentrations;
[0034] Ketraling Capsules (Guangzhou Baiyunshan Pharmaceutical Co., Ltd., batch number: 11100508);
[0035] 1.2 Experimental animals
[0036] SPF-grade Kunming mice, half female and half male, weighing 18-22 g, were provided by the Guangdong Experimental Animal Center, certificate number: SCXK (Guangdong) 2008-0002.
[0037] 2 Experimental methods
[0038] 2.1 Study on the antitussive effect of Dongfengju on mice (concentrated ammonia water spray method)
[0039] Get 98 Kunming mice, divide into 7 grou...
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