Device of pulling and flatting section for pathologic tissue frozen section and method thereof

Abstract

The invention discloses a device of pulling and flatting a section for a pathologic tissue frozen section and a method thereof. The device comprises a water bath box and a section pulling unit, wherein, one side of the water bath box near to the upper margin of the water bath box is provided with a liquid inlet, and one side of the water bath box near to the lower margin is provided with a liquidoutlet; a recyclable or replaceable buffer solution is poured through the liquid inlet and liquid outlet, a support capable of being taken out slowly is hanged in the water bath box, and the level ofthe support is provided with a glass slide sinking to below the liquid level of the buffer solution; the pathologic tissue frozen section is placed in the buffer solution above the glass slide; the section pulling unit has a structure of scissors with handles, and the end portions of the two front forks are connected with metal wires. The method is characterized in that: when pulling the section,the two forks are put in the buffer solution, and the metal wires of the end portions of the two forks are allowed to hold the pathologic tissue frozen section; when flatting the section, the metal wires of the end portions of the two forks of the section pulling unit are separated by the handles of the scissors to flat the pathologic tissue frozen section; then the pathologic tissue frozen section is lightly put on the glass slide, the section pulling unit is pulled out, and finally, the support bearing the glass slide with the section is slowly taken out from the water bath box.

Description

technical field [0001] The invention relates to a device and method for removing and displaying (patching) slices used in biological or medical experiments for making frozen sections of pathological tissues. Background technique [0002] Frozen section is the most widely used method in routine histological preparation techniques. Frozen section is not only used to observe the morphological structure of normal cell tissue, but also the main method used in clinical, scientific research pathology and forensic science to study, observe and judge the morphological changes of cell tissue, and has been widely used in many other disciplines research in the field. In scientific experiments, clinical medicine and experimental teaching, most of the sliced ​​specimens observed under the light microscope are prepared by the frozen section method. Living cells or tissues are mostly colorless and transparent, and there is a lack of contrast between various tissues and various structures ...

AI Technical Summary

Problems solved by technology

And this kind of fishing film is easy to damage the tissue, and the success rate is quite low.

The exhibits are prone to rotten films or overlapping phenomena

Even if it can be stained, the interior of the tissue section is damaged and cannot be effectively observed under the microscope

So far, there is no efficient and simple method of avoiding damage to tissue slices for picking and patching, which has troubled experimenters for a long time and directly affected the experimental process

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