Cellular silk fibroin porous scaffold, and preparation method thereof

A technology of silk fibroin and porous scaffolds, applied in medical science, prostheses, etc., to achieve the effects of avoiding immunogenicity, excellent biocompatibility, and easy availability of raw materials

Inactive Publication Date: 2014-04-23
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application forms of silk fibroin in cell culture are mainly membranes, fibrous porous bodies and porous foams [(a) Perry H, Gopinath A, Kaplan D L, et al. Advanced Materials, 2008, 20: 3070-3072 ; (b) Liu H F, Fan H B, Wang Y, et al.Biomaterials, 2008, 29: 662-674], there are few reports on silk fibroin scaffolds with honeycomb pore structure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Prepare a negative mold sodium chloride porous body with a plant tissue structure. This porous sodium chloride negative mold is prepared as an existing technology. The original plant template is wisteria. For the detailed preparation process, see the invention patent "a honeycomb polymer-based bionic Porous Scaffold Material and Preparation Method” (application number 201010602753.1), which will not be repeated here;

[0025] 2) Preparation of silk fibroin: put silkworm cocoons into 0.5% sodium carbonate aqueous solution, stir at 90°C for 30 minutes, take out, wash with distilled water 4 times, repeat this process 3 times to obtain degummed silk; Dissolve in calcium chloride aqueous solution at 70°C, then dialyze against water in a dialysis bag for 3 days, remove the lower sediment and freeze-dry to obtain silk fibroin;

[0026] 3) Dissolve silk fibroin in anhydrous formic acid to make a silk fibroin solution with a mass fraction of 10%, and use it to impregnate the ...

Embodiment 2

[0029] 1) with the step of embodiment 1;

[0030] 2) Preparation of silk fibroin: Put silkworm cocoons into 0.5% sodium carbonate aqueous solution, stir at 90°C for 10 minutes, take out, wash with distilled water 3 times, repeat this process 3 times to obtain degummed silk; Dissolve in calcium nitrate aqueous solution at 65°C, then dialyze against water in a dialysis bag for 3 days, remove the lower sediment and freeze-dry to obtain silk fibroin;

[0031] 3) Dissolve silk fibroin in hexafluoroisopropanol to make a silk fibroin solution with a mass fraction of 12%, and use it to impregnate the sodium chloride porous body. The specific impregnation method is as follows: the sodium chloride porous body is Press down and immerse in the silk fibroin solution for 0.5 hours, then place it in an environment with a vacuum degree of -4kPa, keep it for 1.5 hours and then raise the vacuum degree to -0.098MPa, keep it for 1 hour and return to normal pressure, take out the sample and vacuum...

Embodiment 3

[0034]1) with the step of embodiment 1;

[0035] 2) Preparation of silk fibroin: Put silkworm cocoons into 0.5% sodium carbonate aqueous solution, stir at 90°C for 30 minutes, take out, wash with distilled water 4 times, repeat this process 3 times to obtain degummed silk; then dissolve it In LiBr aqueous solution at 70°C, then dialyze against water in a dialysis bag for 3 days, remove the lower layer of sediment, and freeze-dry to obtain silk fibroin;

[0036] 3) Making the silk fibroin protein into an anhydrous formic acid solution with a mass fraction of 5% to make a silk fibroin protein solution, and impregnating the sodium chloride porous body with it, the specific impregnation method is as follows: the sodium chloride porous body is placed under normal pressure After soaking in the silk fibroin solution for 0.5 hours, place it in an environment with a vacuum degree of -4kPa, keep it for 1.5 hours and then raise the vacuum degree to -0.098MPa, keep it for 1 hour and retur...

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Abstract

The invention discloses a cellular silk fibroin porous scaffold, and a preparation method thereof. The method comprises the following steps of: degumming silkworm cocoon serving as a raw material, dissolving, dialyzing, and freeze-drying to obtain silk fibroin; and immersing a negative norm sodium chloride porous body which has a plant tissue structure and serves as a template in a silk fibroin solution, vacuum-drying at room temperature, performing beta treatment, and desalting to obtain the silk fibroin porous scaffold which has a bionic plant tissue cellular pore structure. The porosity of the prepared cellar silk fibroin porous scaffold is more than 80 percent, and the apertures of cellular pores are 50 to 300 mu m; and the cellular silk fibroin porous scaffold is high in biocompatibility, and matter permeability, and good in mechanical property and degradation property and the like. The scaffold is particularly suitable for a tissue engineering technology of peripheral nerve, tendon, ligament and the like which have longitudinal structural tissues, and can be widely applied to in-vitro three-dimension cell culture.

Description

technical field [0001] The invention belongs to the field of biomaterials, and in particular relates to tissue engineering technology for peripheral nerves, ligaments, tendons and other tissues with longitudinal topography, in particular to a honeycomb silk fibroin porous scaffold and a preparation method for bionic plant tissue porous structure. Background technique [0002] As an emerging regenerative medicine technology, tissue engineering technology is mainly a type of technology that repairs defective tissues or organs by planting cells on biodegradable three-dimensional porous scaffold materials, followed by in vitro / in vivo culture. Tissue engineering technology has given high hopes to solve the problems of insufficient donor sources and immune rejection in tissue or organ transplantation in clinical practice. [0003] In tissue engineering technology, three-dimensional porous scaffold materials determine the success or failure of tissue engineering technology to a la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/22
Inventor 钱军民金欣霞杨腾飞乔冠军
Owner XI AN JIAOTONG UNIV
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