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Method for extracting exogenous total sugar in dried sea cucumbers

An extraction method and exogenous technology, which is applied in the field of extraction of exogenous total sugars in dried sea cucumbers, can solve the problems of detection result interference, complex processing process, and result interference, and achieve good stability, simple operation, and high accuracy Effect

Inactive Publication Date: 2013-09-18
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

The report of existing measuring sugar content method at present, as the application number is 200910037365.0 " the method for quickly measuring pentose and hexose content in hemicellulose extract liquid ", is to adopt dual-wavelength method to measure total sugar, pentose and hexose content simultaneously, That is, it is suitable for the determination of monosaccharide content, and it is suitable for the samples to be tested with clear ingredients, but the processing process of "sugar-mixed dried sea cucumber" is complicated, and the mixed sugar components are diverse, so this method cannot be used for the determination of the mixed sugar in dried sea cucumbers. Sugar; Determination of Total Sugar Content in Edible Fungus in National Standard GB / T 15672-2009 of the People's Republic of China and GB / T 9695.31-2008 Determination of Total Sugar Content in Meat Products all use spectrophotometry (phenol sulfuric acid method) to determine total sugar content in food. The sugar content has a good reference value, but the methods in the standard are aimed at the determination of the total sugar content in edible fungi and meat products, and are not suitable for the analysis of exogenous total sugar content in sea cucumbers, and cannot meet the requirements for identifying sugar-admixed sea cucumbers
This is because sea cucumber itself has a high protein content and a large amount of polysaccharides, and soluble proteins and polysaccharides will interfere with the test results
The determination of the total sugar content in edible fungi will hydrolyze the polysaccharides of dried sea cucumbers and measure them in the form of added sugars, resulting in misjudgment of the determination results
However, the determination of the total sugar content in meat products has insufficient extraction effect, and water is used as the extraction solvent, and the soluble protein in it will interfere with the results.

Method used

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  • Method for extracting exogenous total sugar in dried sea cucumbers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the mensuration of total sugar content in salt-dried sea cucumber

[0027] The samples were pulverized with a pulverizer and passed through a 40-mesh sieve. Put the sample in a ziplock bag and put it in a desiccator for later use. Weigh about 1g of the sample, accurate to 0.001g, carefully pour it into a 100mL conical flask, add 50mL of 55% ethanol aqueous solution, the volume ratio of sea cucumber sample to ethanol aqueous solution is 1:50, shake and extract in a water bath at 30°C for 1 hour, and cool to room temperature Finally, all the samples and extracts were transferred to a 100mL volumetric flask, rinsed twice, constant volume, filtered, and the filtrate was set aside.

[0028] Accurately draw 1 mL of the above-mentioned filtrate, add 1 mL of 5% phenol aqueous solution, mix well, then quickly add 5 mL of concentrated sulfuric acid vertically, let it stand for 10 min, keep it in a water bath at 30°C for 20 min, and measure the absorbance of the rea...

Embodiment 2

[0037] Embodiment 2: the mensuration of total sugar content in dry sea cucumber

[0038]The sample was pulverized with a pulverizer, passed through a 40-mesh sieve, put the sample in a ziplock bag, and put it in a desiccator for later use. Weigh about 1g of the sample, accurate to 0.001g, carefully pour it into a 100mL conical flask, add 50mL of 55% ethanol aqueous solution, shake and extract in a water bath at 30°C for 1h, cool to room temperature, transfer all the sample and extract to a 100mL volumetric flask, Rinse twice, constant volume, filter, and the filtrate is set aside.

[0039] Accurately draw 1 mL of the above-mentioned filtrate, add 1 mL of 5% phenol aqueous solution, mix well, then quickly add 5 mL of concentrated sulfuric acid vertically, let it stand for 10 min, keep it in a water bath at 30°C for 20 min, and measure the absorbance of the reaction solution at 490 nm (Specrd S100, Analytik Jena Company, Germany) UV-visible spectrophotometer). Obtain the total...

Embodiment 3

[0040] Example 3: Determination of Exogenous Total Sugar Content in Sugar-Added Dried Sea Cucumber

[0041] The sample was pulverized with a pulverizer, passed through a 40-mesh sieve, put the sample in a ziplock bag, and put it in a desiccator for later use. Weigh about 1g of the sample, accurate to 0.001g, carefully pour it into a 100mL conical flask, add 50mL of 55% ethanol aqueous solution, shake and extract in a water bath at 30°C for 1h, cool to room temperature, transfer all the sample and extract to a 100mL volumetric flask, Rinse twice, make up to volume, and filter; in order to prevent the concentration of sugar in the filtrate from being too high, accurately draw 10 mL of the filtrate, transfer it to a 250 mL volumetric flask, make up to volume, and use the filtrate for later use.

[0042] Accurately draw 1 mL of the above filtrate, add 1 mL of 5% phenol aqueous solution, mix well, then quickly add 5 mL of concentrated sulfuric acid vertically, let it stand for 10 m...

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Abstract

The invention provides a method for exogenous total sugar in dried sea cucumbers. The method is characterized by comprising the following steps of: shaking an ethanol aqueous solution with a high concentration under a water bath with a certain temperature to extract exogenous sugar in the sea cucumbers, wherein polysaccharide of the sea cucumbers is not extracted so that endogenous sugar and the exogenous sugar of the sea cucumbers can be better distinguished; and utilizing an optimized phenol-sulfuric acid method to detect the content of the total sugar. The method provided by the invention has the advantages of simplicity in operation, good stability and high accuracy, and is applicable to detecting the content of the exogenous total sugar in the dried sea cucumbers.

Description

Technical field: [0001] The invention relates to the field of extracting total sugars in food, in particular to a method for extracting exogenous total sugars in dried sea cucumbers. Background technique: [0002] Sea cucumber is a nutritious food with high protein content and balanced amino acids and trace elements. It contains about 76.5g of protein, 1.1g of fat, 13.2g of polysaccharide-based sugars, calcium, phosphorus and iron per 100g of pure dry product. , iodine and other beneficial minerals 4.2g. In addition, sea cucumbers also have high medicinal value. According to medical records, sea cucumbers can nourish the kidney and replenish essence, and have certain curative effects on the treatment of tuberculosis hemoptysis, aplastic anemia, diabetes, etc., and have been favored by people for a long time. In the history of our country, sea cucumber is one of the eight treasures of marine products that are as famous as bird's nest and shark's fin. [0003] However, due t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N21/31G01N21/33
Inventor 王联珠殷邦忠李烨宋春丽朱文嘉江艳华姚琳李晓庆郭萌萌谭志军
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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