Method for cultivating and breeding cell tissue of tissue culture seedling of Dendrobium candidum

A technology of dendrobium candidum and cell tissue, applied in the field of cell tissue culture and breeding of dendrobium candidum tissue culture seedlings, can solve the problems of unfavorable preservation of good varieties, separation of characters, etc.

Active Publication Date: 2013-04-17
云南金九地生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the seeds are the product of sexual reproduction, there are genetic differences between the male parent and the female parent, and the offspring bred will have segregation of traits, which is not conducive to the preservation of good varieties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The Dendrobium officinale plant is cleaned with pure water, the leaves are removed, and the outer sheath of the plant is peeled off with tweezers. Soak the plants in 0.1% mercuric chloride solution for disinfection for 15 minutes on the ultra-clean workbench in the clean room. After disinfection, the plants were taken out, rinsed with sterile water, and cut into sections with a sterile scalpel, each section 2.5 cm long. The plant segments were inoculated on the callus culture medium, and cultured at a temperature of 23° C., a humidity of 35%, an illumination of 1800 Lx, and 10 hours of illumination per day. The culture time was 70 days, and the callus grew out. Cut off the callus with a sterile scalpel on an ultra-clean workbench, crush and disperse it with sterile tweezers. The crushed callus was put into the cell culture medium and placed on a shaker for normal temperature culture for 15 days. At the end of the shaker culture, the cells proliferated in the cell cul...

Embodiment 2

[0046] The Dendrobium officinale plant is cleaned with pure water, the leaves are removed, and the outer sheath of the plant is peeled off with tweezers. Soak the plants in 0.1% mercuric chloride solution for disinfection for 15 minutes on the ultra-clean workbench in the clean room. After disinfection, the plants were taken out, rinsed with sterile water, and cut into sections with a sterile scalpel, each section 3.0 cm long. The plant segments were inoculated on the callus culture medium, and cultured at a temperature of 25° C., a humidity of 40%, an illumination of 1800 Lx, and 10 hours of illumination per day. The culture time was 65 days, and the callus grew out. Cut off the callus with a sterile scalpel on an ultra-clean workbench, crush and disperse it with sterile tweezers. The crushed callus was put into the cell culture medium and placed on a shaker for normal temperature culture for 20 days. At the end of the shaker culture, the cells proliferated in the cell cul...

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PUM

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Abstract

A method for cultivating and breeding cell tissue of the tissue culture seedling of Dendrobium candidum comprises the steps of mother plant treatment, soaking disinfection, cleaning and cutting, callus induction, callus crushing, shake cultivation, protocorm cultivation, adventitious bud cultivation, plantlet cultivation and sapling cultivation. According to the invention, good characters of the previous generation are stably inherited by the next generation through a method of asexual reproduction, so that the phenomenon of character segregation of the filial generation, brought by sexual reproduction, can be avoided effectively, the characters of the mother plant can be reserved best, and the production of tissue culture seedling has purposiveness and pertinence.

Description

technical field [0001] The invention relates to a method for cell tissue culture and breeding of dendrobium officinale tissue culture seedlings. Background technique [0002] Dendrobium, also known as Dendrobium orchid, is one of the earliest orchids recorded in ancient Chinese literature. Dendrobium officinale is one of the most valuable varieties of Dendrobium, with extremely high medicinal value. It has been recorded in detail in the pharmaceutical works of all dynasties in my country. "Compendium of Materia Medica" believes that it can eliminate numbness and lower qi, nourish internal organs and strengthen yin Yijing. Take it for a long time, thicken the intestines and stomach, and absolutely replenish the deficiency. Relieves stomach qi, grows muscles, improves intelligence and eliminates shock, lightens body and prolongs life. Dendrobium candidum is listed as the first of the "nine immortal grasses" in China in the Taoist medical classic "Tao Zang". "The Pharmacopoe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 田芳杨永祥李丽陈云
Owner 云南金九地生物科技有限公司
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