Method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling technique

A technology of vancomycin and tobramycin, which is applied in the field of medicine, to achieve the effect of meeting the detection of large batches of samples, reducing the consumption of mobile phase, and improving the efficacy of drugs
CN102539573BActive Publication Date: 2013-04-24ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
Publication Date
2013-04-24

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Abstract

The invention relates to the field of drugs, in particular to a detection method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid. A method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through an ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling techniquecomprises the following steps of: 1) preparing reference samples; 2) preparing internal standard solution; 3) preparing sample solution; 4) preparing mobile-phase solution; 5) setting chromatographicconditions; 6) optimizing mass spectrometric conditions; 7) determining the samples; 8) preparing gradient-concentration reference sample solution; 9) preparing a standard curve; and 10) analyzing and computing data. The method has the characteristics that: 1) the method is advanced; 2) the method is rapid and high-efficiency the loss is low; and 3) the clinical application prospect and the effect are good.
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Description

technical field

[0001] The invention relates to a medicine field, in particular to a detection method for simultaneously measuring the contents of vancomycin and tobramycin in tissue drainage fluid. Background technique

[0002] Vancomycin is a complex glycopeptide antibiotic produced by Streptomyces, with the molecular formula C 66 h 74 C l N 9 o 24 . It is mainly used for systemic and intestinal infections caused by Staphylococcus (including penicillin-resistant and neopenicillin-resistant strains), Clostridium difficile, etc., such as endocarditis, sepsis, and pseudomembranous enteritis. Vancomycin can inhibit the synthesis of bacterial cell walls, and has a strong effect on Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, etc., and has a good effect on Bacillus difficile, Bacillus anthracis, and Bacillus diphtheria. No cross-resistance with other antibiotics, very few drug-resistant strains.

[0003] Tobramycin is an aminoglycoside antibiot...

Claims

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