Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of dendritic cell vaccine loaded with recombinant human gp96-Ki67 antigen peptide compound

A technology of gp96-ki67 and dendritic cells, which is applied in the field of preparation of dendritic cell vaccines, can solve the problems of limited vaccine sources, complicated purification procedures, difficulties in purity and preparation preservation, etc., and achieves the effect of avoiding autoimmune diseases

Inactive Publication Date: 2012-07-11
郑骏年
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the purification process of gp96-peptide complex is complicated and the yield is low, which is difficult to meet the needs of clinical immunotherapy, and there are many difficulties in purity and preparation preservation; secondly, the acquisition, separation and purification of tumor tissues limit the vaccine to a certain extent. source

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The present invention will be further elaborated below by embodiment:

[0022] 1. Preparation of recombinant human gp96 protein and Ki67 antigen CTL epitope peptide

[0023] Recombinant human gp96 protein (rhGrp94) was purchased from ENZO Company, catalog number SPP-766, with a purity > 90%, dissolved in PBS, and the concentration was 1 pmol / μl. The preparation method of the Ki67 antigen CTL epitope peptide used solid-phase synthesis Method, provided by Shanghai Sangong.

[0024] First, connect an amino acid whose amino group is protected by Fmoc group on the insoluble solid phase carrier Wang’s resin, then remove the protecting group of the amino group, and the first amino acid is connected to the solid phase carrier; then protect the amino group by Fmoc group The carboxyl group of the second amino acid is activated with a condensing agent, and the activated amino acid reacts with the amino group of the first amino acid that has been attached to the solid phase carrie...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a dendritic cell vaccine loaded with a recombinant human gp96-Ki67 antigen peptide compound. A preparation method of the dendritic cell vaccine comprises the following steps of: 1, screening, identifying and synthesizing a Ki67 antigen CTL (Cytotoxic T Lymphocyte) epitope peptide; 2, forming the synthesized epitope peptide and a recombinant human heat shock protein gp96 into a compound; and 3, preparing a dendritic cell, and loading the compound onto the dendritic cell to obtain the vaccine finally. The Ki67 antigen peptide can be presented to the dendritic cell through receptor-mediated endocytosis under the assistance of gp96 and cause CTL activation, so that tumor cells expressing the Ki67 antigen are killed. The Ki67 antigen is widely expressed in tumor cells, so that the vaccine has the advantages of wide application range, easiness and high efficiency for preparing, and the like.

Description

technical field [0001] The present invention relates to polypeptide technology in the field of biochemistry and vaccine technology in the field of medical immunology, more specifically, relates to the preparation of a dendritic cell vaccine of a new recombinant human gp96-Ki67 antigen peptide complex. Background technique [0002] Tumor treatment is still a worldwide problem. Traditional treatment methods are difficult to solve tumor recurrence and metastasis after surgery. In 1985, the National Cancer Center of the United States listed tumor biotherapy as the fourth major mode of tumor treatment. In recent years, tumor vaccines have developed rapidly, especially dendritic cell (DC) vaccines are considered to be the most promising therapeutic technology. [0003] To prepare an ideal tumor vaccine, the first step is to select the appropriate tumor antigen. Ki-67 is a nuclear antigen expressed in proliferating cells and is related to cell mitosis. Ki-67 is highly expressed i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P35/00C12N5/0784C07K19/00
Inventor 郑骏年李慧忠李连涛张宝福刘俊杰张青杨洁章龙珍裴冬生
Owner 郑骏年
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com