Recombinant bovine alkaline fibroblast growth factor eye drops
A fibroblast and growth factor technology, applied in the field of recombinant bovine basic fibroblast growth factor eye drops and preparation thereof, to achieve the effects of expanding the scope of application, high patient compliance, and avoiding toxic and side effects
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Embodiment 1
[0035] Embodiment 1, rb-bFGF eye drops and preparation thereof
[0036] Recombinant bovine basic fibroblast growth factor 1680000 IU;
[0037] Human albumin 0.08 g;
[0038] Polyvinyl alcohol 4.0 g;
[0040] Disodium hydrogen phosphate 0.22g;
[0041] Sodium dihydrogen phosphate 0.48g;
[0042] Add water for injection to 400 ml;
[0043] A total of 1000 pieces
[0044] The specific preparation method is:
[0045] (1) Disperse polyvinyl alcohol in an appropriate amount of water for injection at 90°C, autoclave at 121°C, cool to room temperature, and set aside;
[0046] (2) Dissolve recombinant bovine basic fibroblast growth factor, human serum albumin, sodium chloride, disodium hydrogen phosphate and sodium dihydrogen phosphate in an appropriate amount of water for injection, and filter aseptically with a 0.22 μm filter membrane;
[0047] (3) Mix the solution obtained in step (1) and the solution obtained in step (2) evenly under asept...
Embodiment 2
[0049] Embodiment 2, rb-bFGF eye drops and preparation thereof
[0050] Recombinant bovine basic fibroblast growth factor 2,500,000 IU;
[0051] Human albumin 0.12 g;
[0052] Polyvinyl alcohol 2.0 g;
[0053] Sodium chloride 4.0 g;
[0054] Disodium hydrogen phosphate 0.20g;
[0055] Sodium dihydrogen phosphate 0.40g;
[0056] Add water for injection to 400 ml;
[0057] A total of 1000 pieces
[0058] The specific preparation method is:
[0059] (1) Disperse polyvinyl alcohol in an appropriate amount of water for injection at 90°C, autoclave at 121°C, cool to room temperature, and set aside;
[0060] (2) Dissolve recombinant bovine basic fibroblast growth factor, human serum albumin, sodium chloride, disodium hydrogen phosphate and sodium dihydrogen phosphate in an appropriate amount of water for injection, and filter aseptically with a 0.22 μm filter membrane;
[0061] (3) Mix the solution obtained in step (1) and the solution obtained in step (2) evenly under as...
Embodiment 3
[0063] Embodiment 3, rb-bFGF eye drops and preparation thereof
[0064] Recombinant bovine basic fibroblast growth factor 2,000,000 IU;
[0065] Human serum albumin 0.10 g;
[0066] Polyvinyl alcohol 6.0 g;
[0067] Sodium chloride 6.0 g;
[0068] Disodium hydrogen phosphate 0.30g;
[0069] Sodium dihydrogen phosphate 0.50g;
[0070] Add water for injection to 400 ml;
[0071] A total of 1000 pieces
[0072] The specific preparation method is:
[0073] (1) Disperse polyvinyl alcohol in an appropriate amount of water for injection at 90°C, autoclave at 121°C, cool to room temperature, and set aside;
[0074] (2) Dissolve recombinant bovine basic fibroblast growth factor, human serum albumin, sodium chloride, disodium hydrogen phosphate and sodium dihydrogen phosphate in an appropriate amount of water for injection, and filter aseptically with a 0.22 μm filter membrane;
[0075] (3) Mix the solution obtained in step (1) and the solution obtained in step (2) evenly unde...
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