Preparation method of shiga-like toxin Stx1B oral vaccine and product of shiga-like toxin Stx1B oral vaccine

A Shiga-like toxin and oral vaccine technology, applied in the preparation of Shiga-like toxin Stx1B oral vaccine, in the field of Stx1B oral vaccine, can solve the problems of low antigen content and inability to effectively induce intestinal immune response, so as to reduce the incidence rate, The effect of reliable immune effect and simple method
CN102580118AInactive Publication Date: 2012-07-18CHONGQING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHONGQING UNIV
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a shiga-like toxin Stx1B oral vaccine, which concretely comprises the steps of: cloning a shiga-like toxin Stx1B gene, building a recombinant expression vector containing the Stx1B gene, then preparing a transgenic plant, and finally preparing the shiga-like toxin Stx1B oral vaccine. The prepared shiga-like toxin Stx1B oral vaccine can be directly and orally taken to immunize and can effectively prevent the escherichia coli O157:H7 infection, moreover, the orbidity of edema disease of a pig is reduced, and the death efficiency is also reduced at the same time.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, and in particular relates to a preparation method of a Shiga-like toxin Stx1B oral vaccine and the prepared Stx1B oral vaccine. Background technique

[0002] Escherichia coli ( Escherichia coli ) O157: H7 is a serotype of enterohaemorrhagic Escherichia coli. E. coli O157: H7 infection can produce toxins to kill vero cells. The toxin produced is Shiga-like toxin (Stx), which mainly acts on pigs or humans. Colon and ileum cells, the main symptom after infection of pigs is porcine edema disease. Stx contains 1 A subunit and 5 B subunits, forming a complex toxin of A-5B structure. The A subunit is the virulence unit of Stx, which has the activity of RNA-N-terminal glycosidase, which can interfere with the synthesis of intracellular proteins and cause cell damage and death. The B subunit is non-toxic, but it can bind to cells with a specific receptor (Gb3), produce specific antibodies, and then combin...

Claims

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