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Method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines

A technology for fumonisins and traditional Chinese medicines, which is applied in the field of online derivative high-performance liquid phase fluorescence detection, can solve problems such as health hazards, and achieve the effects of accurate results, quick and easy sample processing, and good reproducibility.

Inactive Publication Date: 2012-07-18
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

Therefore, the fumonisins that may exist in these spices and medicinal and edible plants will inevitably bring harm to people's health.

Method used

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  • Method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines
  • Method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines
  • Method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines

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Embodiment Construction

[0124] 1. Determination of fumonisin B in traditional Chinese medicine by on-line derivatization high performance liquid chromatography-fluorescence detection 1 , B 2

[0125] a. Chromatographic conditions: use Synergi C 18 The column (4μm, 250mm×4.6mm) was used for separation, the column temperature was 40°C, the post-column reaction box was used for derivatization (reaction temperature 100°C), and the fluorescence detector was used for detection. The mobile phase was pH 3.15, 0.1 mol / L sodium dihydrogen phosphate solution and methanol, and the flow rate was 0.8 ml / min for gradient elution. At the beginning, it rose from 60% methanol to 85% in 22 minutes; then ran from 85% methanol for 5 minutes, then dropped from 85% methanol to 60% in 0.01 seconds, and finally balanced from 60% methanol for 13 minute. The flow rate of the derivatization reagent was 0.6ml / min, and the reaction volume was 0.6ml. The fluorescence detector has an excitation wavelength of 335 nm and an emis...

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Abstract

The invention relates to a method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines, namely an on-line derivation high-performance liquid phase fluorescent detection method. The method comprises the steps of extracting, purifying and detecting a sample and particularly comprises the following steps of: adding sodium chloride in the sample; performing oscillating extraction by using a horizontal table according to the volume ratio of methanol to water of 4:1; filtering; diluting a certain amount of filtrate by adding water; filtering by using a microfiber filtration membrane; purifying by using an immunoaffinity column; drying by using nitrogen; dissolving by using methanol; and sampling. A derivation agent is prepared by the following steps of: dissolving a proper amount of o-phthaldialdehyde by using methanol; adding into a proper amount of sodium tetraborate solution; adding 2-mercaptoethanol; mixing uniformly; and passing through a microfiltration membrane. The measurement method of the sample comprises the following steps of: separating by using C18 reversed phase chromatographic column; performing gradient elution by using methanol and sodium dihydrogen phosphate as mobile phase; reacting with the derivation agent; detecting by using a fluorescence detector; and confirming the positive sample by using high-performance liquid chromatography-mass spectrum.

Description

Technical field: [0001] The invention relates to a method for simultaneously detecting fumonisin B in different matrix traditional Chinese medicines 1 , B 2 The method, especially the method of on-line derivatized high-performance liquid fluorescence detection, can detect fumonisin B more easily 1 , B 2 . Background technique: [0002] Fumonisins are a group of water-soluble metabolites mainly produced by Fusarium moniliforme. They are a class of mycotoxins with severe cytotoxicity. Among many mycotoxins, they are considered to be one of the more serious threats to humans and livestock. my country has abundant resources of Chinese herbal medicines, and Chinese herbal medicines may be infected with fumonisins during the process of growth, harvesting, processing, and storage. Therefore, it is very necessary to study fumonisins in traditional Chinese medicine, establish a detection method of toxin, and formulate a reasonable limit standard to ensure the safety and effective...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 杨美华谢婷婷
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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