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Material and method for regulating and controlling vernalization of cruciferous plants

A cruciferous, flowering time technology, applied in botanical equipment and methods, angiosperms/flowering plants, plant regeneration, etc., can solve problems such as FLC expression up-regulation

Inactive Publication Date: 2012-08-01
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, FLC expression was found to be upregulated in some mutants

Method used

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  • Material and method for regulating and controlling vernalization of cruciferous plants
  • Material and method for regulating and controlling vernalization of cruciferous plants
  • Material and method for regulating and controlling vernalization of cruciferous plants

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Embodiment Construction

[0036] In the present invention, the term "regulation" refers to artificially controlling the vernalization or flowering time of cruciferous plants by biological means.

[0037] In the present invention, the method of regulation may include overexpressing the sequence shown in SEQ ID NO: 13 or its homologous sequence by using genetic engineering technology, or by transferring the sequence shown in SEQ ID NO: 13 or its homologous sequence to be knocked out or mutated Polynucleotide constructs of homologous sequences, thereby inhibiting their expression and controlling flowering time. Methods for overexpressing and introducing various polynucleotide constructs to knock out or mutate genes are well known in the art.

[0038] In the present invention, cruciferous plants include but not limited to Chinese cabbage, green vegetables, cabbage, radish and the like.

[0039] The present invention includes homologous sequences of SEQ ID NO: 13 found in other Brassicaceae. Homologous se...

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Abstract

The invention relates to a material and method for regulating and controlling the vernalization of cruciferous plants. In particular, the invention relates to a polynucleotides sequence containing a sequence of SEQ ID NO:13, a polynucleotides construct and transformant containing the sequence, a method for regulating and controlling the flowering time of cruciferous plants, and an application of the sequence or construct.

Description

technical field [0001] The invention belongs to the field of regulating the flowering time of cruciferous plants, and in particular relates to substances and methods for regulating the flowering time of cruciferous plants. Background technique [0002] Chinese cabbage (Brassica rapa.pekinensis) is an important vegetable crop, and its vegetative growth stage is divided into four developmental stages: seedling stage, rosette stage, core stage and head stage. This biennial herb needs to go through a winter to bloom. After the plant is treated with low temperature, it blooms in the second spring. This process is called vernalization. [0003] During any period of vegetative growth of Chinese cabbage, as long as there is a low temperature environment (below 10°C) for 20-50 days, the plants can enter the reproductive growth stage through vernalization. In terms of production, "immature bolting" is a major problem that causes yield and quality loss of Chinese cabbage. ball. In o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/63C12N5/10A01H4/00A01H5/00
Inventor 何玉科张绍峰孙传宝
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI