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Soybean growth period E1 gene and encoding protein of soybean growth period E1 gene

A technology for encoding protein and growth period, which can be used in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc.

Inactive Publication Date: 2013-05-08
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the E4, E3, Dt1 and E2 genes have been cloned successively by the model crop homologous gene method or the fine map mapping method, there has been no report on the successful cloning of the E1 gene so far.

Method used

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  • Soybean growth period E1 gene and encoding protein of soybean growth period E1 gene
  • Soybean growth period E1 gene and encoding protein of soybean growth period E1 gene
  • Soybean growth period E1 gene and encoding protein of soybean growth period E1 gene

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specific Embodiment approach 1

[0026] Specific embodiment 1: A soybean growth stage E1 gene sequence according to this embodiment is shown in the sequence table Seq ID No: 1.

specific Embodiment approach 2

[0027] Embodiment 2: The amino acid sequence of the protein encoded by the soybean growth stage E1 gene of this embodiment is shown in Seq ID No: 2 in the sequence table.

[0028] The methods for obtaining and identifying the E1 gene in soybean growth stage are as follows:

[0029] (1) Acquisition of soybean growth stage E1 gene

[0030] 1. In 2004, the two near-allelic lines of soybean Harosoy-E1 (L68-694, E1e2E3E4e5, PI 547707) and Harosoy-e1 (L58-266, e1e2E3E4e5, PI 547676) were crossed, and then by map position The cloning method was used to screen the E1 gene; 2. Among the 117 F2 generation soybean populations after hybridization, the E1 gene locus was screened between Satt365 and Satt489 by the map-based cloning method; 3. Further 1442 F2:3 Screened the soybean population of the first generation, and screened out 7 genetic recombinants in the located interval. Among them, the E1 gene loci of the 7 screened genetic recombinants were located between Satt365 and Satt557, a...

specific Embodiment approach 3

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Abstract

The invention discloses a soybean growth period E1 gene and encoding protein of the soybean growth period E1 gene and relates to the soybean growth period E1 gene and the encoding protein of the soybean growth period E1 gene. The invention provides the soybean growth period E1 gene and the encoding protein of the soybean growth period E1 gene. The soybean growth period E1 gene sequence is shown as a sequence table sequence identification number (Seq ID No):1. The amino acid sequence of the encoding protein of the soybean growth period E1 gene is shown as the Seq ID No:2. The E1 gene is clonedby a map-based cloning method, and the physiological function of the E1 gene for intensively inhibiting the soybean flowering is verified through transgenosis and screening of a plurality of ethyl methane sulfonate (EMS) mutant libraries. The invention relates to the field of soybean gene.

Description

technical field [0001] The invention relates to a soybean growth stage E1 gene and its encoded protein. Background technique [0002] Soybeans provide human beings with important vegetable proteins and oils. Worldwide, soybeans are cultivated in a wide range of regions ranging from high latitudes in northern Europe, Sweden and North America to Canada, and south to Brazil and Argentina. It is closely related to the growth period gene of soybean. Nine gene loci E1-E8 and J that mainly affect soybean flowering and maturity (growth) stages have been discovered so far. As early as the 1920s, American scholar Owen discovered a pair of main genetic factors controlling soybean ripeness, and named them E and e. Later, Bernard (1971) speculated that E and e were different alleles of the same locus, and named them E1 / e1. The contribution rate of E1 locus to flowering stage and mature stage is as high as 50%-70%. The soybean growth period gene E1 not only has the greatest impact on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415
Inventor 夏正俊翟红吕世翔吴红艳
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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