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Gene capable of inhibiting proliferation of gastric cancer cell bgc-823 and preparation method thereof

A technology for BGC-823 and gastric cancer cells, applied in gene therapy, DNA preparation, pharmaceutical formulations, etc., can solve problems such as unsatisfactory overall curative effect

Inactive Publication Date: 2014-10-01
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although comprehensive measures such as surgery, chemotherapy and radiotherapy are used to treat gastric cancer, the overall curative effect is still unsatisfactory.

Method used

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  • Gene capable of inhibiting proliferation of gastric cancer cell bgc-823 and preparation method thereof
  • Gene capable of inhibiting proliferation of gastric cancer cell bgc-823 and preparation method thereof

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Experimental program
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Embodiment Construction

[0020] 1. Culture of gastric cancer cell BGC-823: Aspirate the culture medium in the culture dish full of cells, add 2ml of sterile 1×D-Hanks to wash twice, discard the washing solution, add 0.25% trypsin 1-1.5 ml, The digestion solution evenly covers the cell surface, and the digestion time is about 1-3 minutes. After digestion, it can be seen that the cells shrink and become round under the inverted microscope, and the refraction of the edge is enhanced. When the cells have not fallen off, the digestion solution is poured out, and serum or serum-containing medium is added immediately to terminate the digestion. Blow off the cells on the wall of the culture flask, centrifuge at 1000 rpm / min for 5 minutes, discard the medium, add 6ml of RPMI-1640 culture solution containing 10% FBS, mix well by blowing and blowing, depending on the cell density, the ratio is 1:2 or 1 : Cultivate in 3 separate dishes, when the cells fill the culture flask and form a dense monolayer, they can be...

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Abstract

The invention relates to a gene capable of inhibiting proliferation of gastric cancer cell BGC-823 and a preparation method thereof. The gene is two partially complementary nucleotide sequences of Stat5b-shRNA designed according to gastric cancer cell BGC-823, and then the two complementary nucleotide sequences are connected with the SilenCircle plasmid in the commercially available SilenCircleTMRNAi transcription kit to obtain A gene that has the function of inhibiting the proliferation of gastric cancer cell BGC-823. The invention designs the RNAi of genes associated with the proliferation of gastric cancer cell BGC-823, and uses the gastric cancer cell BGC-823 model to study its inhibitory effect on the proliferation of gastric cancer cell BGC-823. It provides a new method for the application of RNAi in the treatment of gastric cancer.

Description

technical field [0001] The invention relates to a gene capable of inhibiting proliferation of gastric cancer cell BGC-823 and a preparation method thereof. Background technique [0002] Gastric cancer is one of the most common malignant tumors in my country, and its morbidity and mortality rank the top two among malignant tumors in my country. Due to the lack of specific screening indicators for gastric cancer and the lack of obvious symptoms in early stage patients, it is difficult to find early gastric cancer, and most gastric cancer patients have already entered the advanced stage when they see a doctor. At present, although comprehensive measures such as surgery, chemotherapy and radiotherapy are used to treat gastric cancer, the overall curative effect is still unsatisfactory. With the deepening of research on molecular biology of gastric cancer, molecular targeted therapy aimed at molecular biological targets such as tumor cell growth, apoptosis, cell cycle, invasion ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/10A61K48/00A61P35/00
Inventor 陈媛媛朱静嬿李哲武岳余抗抗宋一超陈付学
Owner SHANGHAI UNIV