Quantitative detection reagent and quantitative detection method for urinary protein liquid

A quantitative detection method and quantitative detection technology, applied in the biological field, can solve the problems of false positives, many factors affected by the dry chemical test paper method, false negatives, etc., and achieve the effect of rapid operation

Inactive Publication Date: 2012-09-19
JIAXING JIUQIJIU BIOLOGICAL TECH
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Problems solved by technology

[0005] The urine test technology currently used in China is mainly the qualitative test system of dry chemical test paper, which can only provide qualitative analysis results such as negative or positive, and cannot be quantitative
In addition, the dry chemical test paper method is also affected by many factors, and false negatives and false positives will inevitably occur in clinical work, making it difficult to provide accurate diagnostic information for clinicians

Method used

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  • Quantitative detection reagent and quantitative detection method for urinary protein liquid
  • Quantitative detection reagent and quantitative detection method for urinary protein liquid

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Experimental program
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Embodiment 1

[0030] Embodiment 1: Preparation steps of a urine protein liquid quantitative detection reagent:

[0031] a. Add 0.4g of pyrophenol into 0.5L of organic solvent, and stir until completely dissolved to form a pyrophenol solution;

[0032] b. Dissolve the following compounds with 0.4L distilled water: 0.5g succinic acid, 0.09g sodium benzoate, 0.06g sodium molybdate, completely dissolve to obtain an intermediate solution;

[0033] c. Mix the intermediate solution with the pyrogol solution, stir and mix evenly, and then set the volume to 1 L to form a urine protein liquid quantitative detection reagent.

[0034] Example 2 3 4 5 6 7 8 9 10 11 12 13 Organic solvent (L) 0.1 0.1 0.2 0.2 0.35 0.35 0.5 0.5 0.6 0.6 0.7 0.7 Pyrophenol (g) 0.1 0.1 0.35 0.35 0.35 0.4 0.4 0.4 0.7 0.7 1.0 1.0 Succinic acid (g) 0.1 0.1 0.1 0.1 0.25 0.25 0.25 0.5 0.5 0.5 0.5 0.5 Sodium benzoate (g) 0.01 0.01 0.03 0.03 0.06 0.06...

Embodiment 14

[0035] Embodiment 14: A quantitative detection method of urine protein liquid reagent, the urine protein liquid quantitative detection method of the present invention is based on a kind of biochemical reaction principle, when protein in urine and pyrogallol red in liquid detection reagent When the molybdenum complex is in contact, the amino group of the protein can form a blue compound with it, which has an absorption peak at a wavelength of 600nm. The concentration of protein or quality control in urine can be quantified by making a standard curve of protein concentration versus absorbance dose effect. The higher the protein content in the sample, the stronger the signal from the absorbance value.

[0036] The urine protein liquid quantitative detection method of any reagent of the present invention comprises the following steps:

[0037] a. Put the urinary protein liquid quantitative detection reagent into the R1 reagent compartment of the automatic analyzer Olympus5400 aft...

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Abstract

The invention discloses a quantitative detection reagent and a quantitative detection method for urinary protein liquid. The reagent is obtained by mixing pyrogallol solution with an intermediate solution. The intermediate solution is obtained by dissolving a certain amount of succinic acid, sodium benzoate and sodium molybdate in distilled water. By combining an automatic analyzer with the quantitative detection reagent for the urinary protein liquid, quantitative and accurate content of protein in a urine sample can be provided, so that accurate information can be provided to clinical doctors, and quantitative, automatic and fast detection of the urine sample can be achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a urine protein (Urinary Protein) liquid quantitative detection reagent and method. Background technique [0002] Urine is formed by the filtration of certain blood components by the glomeruli, reabsorption, collection, and excretion by the renal tubules. Clinical urine testing can help doctors understand the changes in patients' urinary physiological and pathological functions, and can also indirectly reflect the function of multiple organs and systems throughout the body. [0003] Proteinuria refers to a condition in which the urine contains abnormally high concentrations of protein. The urinary protein is mainly albumin, but also contains some globulin and protein secreted by nephrons. Under normal circumstances, most proteins cannot enter the urine through the filtration of the glomeruli, but when the kidney function is damaged, some proteins will enter the urine, forming proteinu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31
Inventor 虞留明蔡江丽田军孟雷
Owner JIAXING JIUQIJIU BIOLOGICAL TECH
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