Separation preparation process for high-purity irisflorentin

A technology of irisin and preparation process, which is applied in the field of separation and preparation of high-purity irisin, can solve the problems of large equipment investment, inability to enlarge the process, complicated solvent system, etc. good purity

Inactive Publication Date: 2012-10-03
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the Chinese patent "Extract of Shegan Total Flavonoids and Its Preparation Method and Its Application in Drug Preparation" (Application No. 200510056727.2), the method of enriching the macroporous resin was used to prepare the total flavonoids of Shegan. This method did not obtain pure irisaxanthin
In the Chinese patent "Secondary High-speed Countercurrent Chromatography Separation Method for Is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Get 1kg of white flower shoots and put it into the extraction kettle, with supercritical CO 2 As a solvent, the supercritical extraction of white flowers is carried out, and the extraction temperature is 65 ° C, the pressure is 22 MPa, and the CO 2 The flow rate is 20L / h. After the environment in the extraction tank is stable, add 90% acetone carrier (90% of the amount of medicinal materials) to the carrier storage tank, and after 5h of cyclic extraction, adjust the temperature of the analysis kettle to 65 °C and the pressure to be 10MPa, collect the extract released from the discharge port of the analysis kettle. Add ethanol and ammonium sulfate aqueous two-phase system to the extract in proportion to heat and mix, let stand, divide into upper and lower phases, take out the ethanol phase rich in flavonoids, add ethyl acetate, water and sodium chloride, mix well, Take the upper phase and concentrate under reduced pressure to obtain crude irisin. The crude product was ...

Embodiment 2

[0018] Get 1kg of white flower shoots and put it into the extraction kettle, with supercritical CO 2 As a solvent, the supercritical extraction of white flowers is carried out, and the extraction temperature is 30 ° C, the pressure is 45 MPa, and the CO 2 The flow rate is 50L / h. After the environment in the extraction tank is stabilized, add 65% acetone carrier (50% of the amount of medicinal materials) to the carrier storage tank, and after 2h of cyclic extraction, adjust the temperature of the analysis kettle to 40 °C and the pressure to be 4MPa, collect the extract released from the discharge port of the analysis kettle. Add ethanol and ammonium sulfate aqueous two-phase system to the extract in proportion to heat and mix, let stand, divide into upper and lower phases, take out the ethanol phase rich in flavonoids, add ethyl acetate, water and sodium chloride, mix well, Take the upper phase and concentrate under reduced pressure to obtain crude irisin. The crude product w...

Embodiment 3

[0020] Get 5kg of white flower shoots and put it into the extraction kettle, with supercritical CO 2 As a solvent, supercritical extraction of white flowers is carried out, and the extraction temperature is 45 ° C, the pressure is 38 MPa, and the CO 2 The flow rate is 40L / h. After the environment in the extraction tank is stable, add 80% acetone carrier agent (70% of the amount of medicinal materials) into the carrier agent storage tank, and after 4h of cyclic extraction, adjust the temperature of the analysis kettle to 55 °C and the pressure to be 8MPa, collect the extract released from the discharge port of the analysis kettle. Add ethanol and ammonium sulfate aqueous two-phase system to the extract in proportion to heat and mix, let stand, divide into upper and lower phases, take out the ethanol phase rich in flavonoids, add ethyl acetate, water and sodium chloride, mix well, Take the upper phase and concentrate under reduced pressure to obtain crude irisin. The crude pro...

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PUM

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Abstract

The invention discloses a separation preparation process for high-purity irisflorentin. The separation preparation process comprises the following steps of: extracting supercritical CO2 to obtain flavonoid substances; extracting and enriching a solvent to obtain crude products of the irisflorentin; separating by means of preparation type reversed high performance liquid chromatography; and purifying to obtain crystals of the irisflorentin. According to the separation preparation process, flavonoid compounds in vesper iris herb can be continuously extracted, and high-purity irisflorentin monomers can be prepared.

Description

technical field [0001] The invention belongs to the technical field of extraction of effective components of traditional Chinese medicines, and relates to a separation and preparation process of high-purity irisaxanthin. Background technique [0002] Baihuashegan (Iris dichotoma Pall.) is the rhizome or whole plant of Iris dichotoma Pall., a perennial herb. The whole plant is harvested in spring, and the rhizome is harvested in autumn. It is used fresh or cut into sections and dried in the sun. It mostly grows in sunny and dry places such as sandy grasslands and rock crevices on hillsides, and is mainly distributed in Northeast China, North China, Shaanxi, Ningxia, Gansu, Qinghai, Shandong, Anhui, Jiangsu, Jiangxi, Henan and other places. Radix Bacteriae is bitter, pungent, cold in nature, slightly poisonous, and returns to the lung, stomach, and liver meridian. Indications for sore throat, gingival swelling and pain, mumps, mastitis, stomach pain, hepatitis, hepatosplenome...

Claims

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Application Information

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IPC IPC(8): C07D493/04
CPCY02P20/54
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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