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A preparation method of dopamine-modified hyaluronic acid micelles

A technology of hyaluronic acid and dopamine, applied in the field of polymer materials and natural macromolecules, to achieve the effect of short reaction period, excellent biocompatibility, and unique moisturizing type

Active Publication Date: 2017-09-05
林玉洪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the use of this excellent adhesion of dopamine to introduce it into natural macromolecules to optimize emulsification to a certain extent.

Method used

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  • A preparation method of dopamine-modified hyaluronic acid micelles
  • A preparation method of dopamine-modified hyaluronic acid micelles
  • A preparation method of dopamine-modified hyaluronic acid micelles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of hyaluronic acid graft

[0030] Weigh 2g with a molecular weight of 3.1×10 5 Da hyaluronic acid (HA), put into a 250mL single-necked flask, add 80mL deionized water, stir and dissolve at room temperature for 1h after vacuumizing. After complete dissolution, adjust the pH of the solution to 5.5, add 1.91g of EDC, 1.15g of NHS and 1.35g of HOBt, and stir for 1h to activate the carboxyl group. In addition, 1.8 g of dopamine hydrochloride (DOPA) was weighed, put into a flask containing HA, vacuumized for 15 min, and reacted at room temperature for 24 h. The reaction solution was dialyzed in deionized water for 4 to 6 days to remove the catalyst and unreacted monomers, and an ultraviolet spectrometer was used to track the absorption peak of dopamine in the dialysate at around 280 nm until the absorbance was 0, then freeze-dried to obtain the grafted product HA-DOPA.

[0031] (2) Preparation of hyaluronic acid micelles

[0032] Weigh 0.5g HA-DOPA, dissolv...

Embodiment 2

[0036] (1) The preparation of the hyaluronic acid graft was the same as in Example 1.

[0037] (2) The preparation of hyaluronic acid micelles is the same as in Example 1.

[0038] (3) Preparation of emulsion

[0039] Adjust a series of 3 mL of the above micellar aqueous solution with different pH, add an equal volume of white oil, and mix and homogenize at 8000 rpm for 2 minutes to obtain a series of emulsions. The emulsification effect of the emulsion can be well controlled by the acidity and alkalinity of the system.

Embodiment 3

[0041] (1) Preparation of hyaluronic acid graft

[0042] Weigh 2g of hyaluronic acid (HA), put it into a 250mL single-necked flask, add 80mL of deionized water, stir and dissolve at room temperature for 1h after vacuuming. After complete dissolution, adjust the pH of the solution to 5.5, add 1.91g of EDC, 1.15g of NHS and 1.35g of HOBt, and stir for 1h to activate the carboxyl group. In addition, 9 g of dopamine hydrochloride (DOPA) was weighed, put into a flask containing HA, vacuumized for 15 min, and reacted at room temperature for 24 h. The reaction solution was dialyzed in deionized water for 4 to 6 days to remove the catalyst and unreacted monomers, and the absorption peak of dopamine in the dialysate at around 280nm was tracked with an ultraviolet spectrometer until the absorbance was 0, then freeze-dried to obtain the grafted product HA-DOPA .

[0043] (2) The preparation of hyaluronic acid micelles is the same as in Example 1.

[0044] (3) Preparation of emulsion ...

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Abstract

The invention provides a method for preparing dopamine-modified hyaluronic acid micelles, which belongs to the technical field of polymer materials and natural macromolecules. The invention introduces a dopamine unit into the main chain of the hyaluronic acid through amidation reaction, endows the hyaluronic acid with a certain amphiphilicity, and self-assembles in an aqueous solution to form micelles with particle diameters in the range of 100-700nm. This hyaluronic acid micelle has excellent biocompatibility, hydrophilicity and viscoelasticity, and can be used in the fields of biomaterials, hydrophilic colloids, etc., and the adhesion of dopamine endows it with good interfacial properties, which is used for emulsification Agents can be used in emulsion, food and cosmetics and other fields.

Description

technical field [0001] The present invention relates to a preparation method of dopamine-modified hyaluronic acid micelles. Dopamine units are introduced into the main chain of hyaluronic acid, and micelles with emulsifying properties are obtained through self-assembly of hyaluronic acid in aqueous solution, which belongs to high Molecular materials and natural macromolecules technology field. Background technique [0002] In almost all practical emulsions, some kind of emulsifier needs to be added to form a stable emulsion, and the presence of emulsifier is crucial to the formation and stability of the emulsion. Surfactants, macromolecular substances and solid particles can be used as emulsifiers and stabilizers for emulsions. Among them, the emulsion formed by solid particles adsorbed on the oil / water interface to stabilize the emulsion droplets is called Pickering emulsion, and the emulsifier used is called particle emulsifier. Compared with traditional small molecule s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/09C08J3/03C08L5/08C08B37/08
Inventor 刘晓亚王娟勤白绘宇易成林谭新强
Owner 林玉洪
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