A kind of preparation method of extracellular whole protein extract for controlling Monochamus alternata
A technology of Monochamus alternatus and whole protein, which is applied in the field of preparation of extracellular whole protein extract, and can solve problems such as ineffective effects
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[0018] Now in conjunction with the drug efficacy test of the present invention, further illustrate the beneficial effects of the present invention.
[0019] The preparation method of the extracellular whole protein extract of the control Monochamus alternatus comprises the following steps:
[0020] 1) Inoculate the Beauveria bassiana strain on PPDA medium, culture it in an incubator at 23°C for 16 days, scrape off the conidia, and dilute it with sterile water to 1.0×10 8 pcs mL - 1 The spore suspension was inoculated on the fermentation medium according to the spore suspension: fermentation medium volume ratio of 1:100, and then it was placed in a constant temperature shaking incubator at 25°C and 130rpm to cultivate for 7d, for subsequent use;
[0021] 2) Take the fermentation broth from step 1, centrifuge at 4°C and 4000rpm for 13 minutes, collect the supernatant, and vacuum filter it with 3 layers of filter paper to remove the mycelium suspended in the fermentation broth,...
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