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Sea cucumber glycopeptide, preparation method and application to cosmetic preparation

The technology of sea cucumber glycopeptide and sea cucumber is applied to the application field of sea cucumber glycopeptide and preparation of cosmetics, and achieves the effects of safe and reliable raw material source, wide raw material source and simple preparation process.

Active Publication Date: 2014-12-31
源美生物技术开发(大连)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report about the preparation of sea cucumber glycopeptides by enzymatic hydrolysis and its application in cosmetics using sea cucumbers as raw materials

Method used

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  • Sea cucumber glycopeptide, preparation method and application to cosmetic preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method is carried out as follows:

[0020] a. Homogenize the sea cucumber body wall at 10°C;

[0021] b. Add 0.01 to 0.1M phosphate buffer solution with 3 times volume of pH 7 to 8 and a compound enzyme with a mass ratio of 0.1% to the obtained homogenate, and perform enzymatic hydrolysis for 48 h; the compound enzyme is alkaline protease, A mixture of papain and trypsin in the ratio of activity units of 3:3:4;

[0022] c. Take the supernatant by centrifugation, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 3000 Da or less, and take the liquid that passes through the ultrafiltration membrane;

[0023] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;

[0024] e. Pass the collected liquid through the macroporous adsorption resin (HPD system List ) after desalination and activated carbon and freeze-drying to obtain sea cucumber glycopeptides.

[0025] T...

Embodiment 2

[0027] The preparation method is carried out as follows:

[0028] a. Homogenize the sea cucumber body wall at 0°C;

[0029] b. Add 0.01-0.1M phosphate buffer solution with 1 volume of pH 7-8 to the obtained homogenate and a compound enzyme with a mass ratio of 1% to the homogenate, and perform enzymatic hydrolysis for 12 h; the compound enzyme is alkaline protease, A mixture of papain and trypsin in the ratio of activity units of 3:3:4;

[0030] c. Take the supernatant by centrifugation, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 3000 Da or less, and take the liquid that passes through the ultrafiltration membrane;

[0031] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;

[0032] e. Pass the collected liquid through the macroporous adsorption resin (HPD system List ) after desalination and activated carbon and freeze-drying to obtain sea cucumber glycopeptides.

[0033...

Embodiment 3

[0035] The preparation method is carried out as follows:

[0036] a. Homogenize the sea cucumber body wall at 5°C;

[0037] b. Add 0.01-0.1M phosphate buffer solution with 1 volume of pH 7-8 to the obtained homogenate and a compound enzyme with a mass ratio of 0.5% to the homogenate, and perform enzymatic hydrolysis for 30 h; the compound enzyme is alkaline protease, A mixture of papain and trypsin in the ratio of activity units of 3:3:4;

[0038] c. Take the supernatant by centrifugation, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 3000 Da or less, and take the liquid that passes through the ultrafiltration membrane;

[0039] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;

[0040] e. Pass the collected liquid through the macroporous adsorption resin (HPD system List ) after desalination and activated carbon and freeze-drying to obtain sea cucumber glycopeptides.

[00...

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Abstract

The invention discloses a sea cucumber glycopeptide. The sea cucumber glycopeptide is characterized in that the molecular weight is less than 3000 Da, and a carbohydrate chain is connected onto serine or threonine through GalNAc to form a structure of -Galbeta1-3GalNAc alpha-O-Ser / Thr. The sea cucumber glycopeptide is prepared from sea cucumber by means of enzymolysis and the like, is wide, safe and reliable in source of raw materials, simple in preparation method, low in cost and convenient to apply. The prepared sea cucumber glycopeptide has functions of remarkably promoting growth of fibroblasts and protecting injury of the fibroblasts caused by ultraviolet radiation, can be applied to cosmetic preparation and can effectively prevent ultraviolet injury, so that skin aging resulting from ultraviolet irradiation and the like is avoided.

Description

technical field [0001] The invention relates to a sea cucumber enzymatic hydrolyzate, in particular to a sea cucumber glycopeptide which has a simple preparation process, low cost, significant effect of promoting fibroblast growth and protecting fibroblasts from ultraviolet radiation damage, a preparation method and preparation method. Application in cosmetics. Background technique [0002] At present, there are many kinds of sunscreen cosmetics, and their sunscreen principles are different. For example, the Chinese invention patent number is ZL02817885.8, and the invention patent named "Silicon dioxide-coated mixed crystal oxide particles, its preparation method and cosmetic materials prepared therefrom" discloses a sunscreen (prevention of skin damage caused by ultraviolet rays) ) functional cosmetics are made by adding silicon dioxide-coated mixed crystal oxide particles with the ability to block ultraviolet rays to the basic raw materials of cosmetics, that is, to preve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61Q17/04
Inventor 李伟佟长青曲敏成芳金桥
Owner 源美生物技术开发(大连)有限公司