Sea cucumber glycopeptide, preparation method and application to cosmetic preparation
A technology for cosmetics and sea cucumber, which is applied in the preparation of cosmetics, in the field of sea cucumber glycopeptides, achieves the effects of simple preparation process, safe and reliable source of raw materials, and avoiding skin aging.
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Embodiment 1
[0019] The preparation method is carried out as follows:
[0020] a. Homogenize the sea cucumber body wall at 10°C;
[0021] b. Add 0.01 to 0.1M phosphate buffer solution with 3 times volume of pH 7 to 8 and a compound enzyme with a mass ratio of 0.1% to the obtained homogenate, and perform enzymatic hydrolysis for 48 h; the compound enzyme is alkaline protease, A mixture of papain and trypsin in the ratio of activity units of 3:3:4;
[0022] c. Take the supernatant by centrifugation, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 3000 Da or less, and take the liquid that passes through the ultrafiltration membrane;
[0023] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;
[0024] e. Pass the collected liquid through the macroporous adsorption resin (HPD system List ) after desalination and activated carbon and freeze-drying to obtain sea cucumber glycopeptides.
[0025] T...
Embodiment 2
[0027] The preparation method is carried out as follows:
[0028] a. Homogenize the sea cucumber body wall at 0°C;
[0029] b. Add 0.01-0.1M phosphate buffer solution with 1 volume of pH 7-8 to the obtained homogenate and a compound enzyme with a mass ratio of 1% to the homogenate, and perform enzymatic hydrolysis for 12 h; the compound enzyme is alkaline protease, A mixture of papain and trypsin in the ratio of activity units of 3:3:4;
[0030] c. Take the supernatant by centrifugation, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 3000 Da or less, and take the liquid that passes through the ultrafiltration membrane;
[0031] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;
[0032] e. Pass the collected liquid through the macroporous adsorption resin (HPD system List ) after desalination and activated carbon and freeze-drying to obtain sea cucumber glycopeptides.
[0033...
Embodiment 3
[0035] The preparation method is carried out as follows:
[0036] a. Homogenize the sea cucumber body wall at 5°C;
[0037] b. Add 0.01-0.1M phosphate buffer solution with 1 volume of pH 7-8 to the obtained homogenate and a compound enzyme with a mass ratio of 0.5% to the homogenate, and perform enzymatic hydrolysis for 30 h; the compound enzyme is alkaline protease, A mixture of papain and trypsin in the ratio of activity units of 3:3:4;
[0038] c. Take the supernatant by centrifugation, separate with an ultrafiltration membrane separator with a molecular weight cut-off of 3000 Da or less, and take the liquid that passes through the ultrafiltration membrane;
[0039] d. Collect OD by Sephadex LH-20 molecular sieve chromatography column (2.5×60~100cm) 280nm absorption peak;
[0040] e. Pass the collected liquid through the macroporous adsorption resin (HPD system List ) after desalination and activated carbon and freeze-drying to obtain sea cucumber glycopeptides.
[00...
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