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Zein nano embedded slow-release filler and preparation method thereof

A zein and gliadin technology, which is applied in directions such as drug delivery, drug combination, and pharmaceutical formulation, can solve the problems of complex preparation process, poor drug stability, side effects of organisms, etc., and achieves simple operation, high biological efficiency and the like. Compatibility, source safe and reliable effect

Active Publication Date: 2018-01-19
云智前沿科技发展(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The emulsification method is currently a common method for preparing drug embeddings, but in the preparation process, it is necessary to add a variety of additives such as organic solvents and surfactants. The reactions of various organic substances are not only complicated and difficult to control, but also these The residue of the reagent will not only affect the activity of the drug, but even cause side effects on the organism; in addition, there are also methods of desolvation and chemical cross-linking to prepare drug embedding, but it also involves uncontrollable multi-substance reactions and toxic cross-linking agents usage of
In addition, the above-mentioned methods are not only complicated in preparation process, but also often have low drug loading and coating rate, uneven drug distribution and easy agglomeration, and poor drug stability, which affects the therapeutic effect, so it is not suitable for preparing drug embedding. ideal technology

Method used

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  • Zein nano embedded slow-release filler and preparation method thereof

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preparation example Construction

[0027] The preparation method of the zein nano-embedded slow-release filler of the present invention includes the steps of dissolving the prolamin, dissolving the antitumor drug, mixing the solution, and concentrating and drying. The specific steps are as follows:

[0028] A, prolamin dissolution: take zein and add it to ethanol aqueous solution and stir until the solution is uniform and transparent to obtain a zein solution;

[0029] B, antineoplastic drug dissolution: take antineoplastic drug, add ethanol aqueous solution or water and stir until the solution is uniform and transparent, obtain antineoplastic drug ethanol aqueous solution or antineoplastic drug aqueous solution;

[0030] C. Solution mixing: mix the above-mentioned antineoplastic drug ethanol solution or antineoplastic drug aqueous solution with the zein solution to obtain a zein nano-embedding mixed solution;

[0031] D. Concentration and drying: freeze-dry or spray-dry the above-mentioned zein nano-embedded m...

Embodiment 1

[0041] 1. Under normal temperature and pressure, weigh 30mg of zein, dissolve it in 30ml of 80% (about 25.8g, density 0.85932 g / ml) ethanol-water solution, and stir it ultrasonically for 8-12min at normal temperature and pressure Until the solution is uniform and transparent, it is prepared into a 1 mg / ml zein solution.

[0042]2. At normal temperature and pressure, weigh 9 mg of doxorubicin, dissolve it in 30 ml of 80% (about 25.8 g, density 0.85932 g / ml) ethanol-water solution, and stir it ultrasonically for 8 to 12 minutes at normal temperature and pressure Until the solution is uniform and transparent, it is prepared into a 0.3 mg / ml ethanol solution of doxorubicin.

[0043] 3. Immediately mix the 0.3 mg / ml doxorubicin ethanol aqueous solution with the 1 mg / ml zein solution to obtain a zein nano-embedding mixed solution;

[0044] 4. Put the above-mentioned zein nano-embedding mixture in a petri dish and freeze-dry to obtain the zein nano-embedded slow-release filler.

Embodiment 2

[0046] 1. At normal temperature and pressure, weigh 30 mg of zein, dissolve it in 60 ml of 60% (about 54.5 g, density 0.90916 g / ml) ethanol-water solution, and stir it ultrasonically for 8 to 12 minutes at normal temperature and pressure Until the solution is uniform and transparent, it is prepared into a 0.5 mg / ml zein solution.

[0047] 2. Under normal temperature and pressure, weigh 15mg of 5-fluorouracil, dissolve it in 30ml of 60% (about 27.3g, density 0.90916g / ml) ethanol-water solution, and stir it ultrasonically for 8-12min at normal temperature and pressure. The solution is homogeneous and transparent, and is prepared as 0.5 mg / ml 5-fluorouracil ethanol aqueous solution.

[0048] 3. Immediately mix the 0.5 mg / ml 5-fluorouracil ethanol aqueous solution with the 0.5 mg / ml zein solution to obtain a zein nano-embedding mixed solution;

[0049] 4. Distilling the above zein nano-embedded mixed solution under reduced pressure to obtain a concentrated solution with a solid c...

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Abstract

The invention discloses a zein nano embedded slow-release filler and a preparation method thereof. The zein nano embedded slow-release filler is prepared from the following raw materials in parts by weight: 5-15 parts of zein and 2-10 parts of an antitumor drug. The preparation method comprises the following steps: adding zein into an aqueous solution of ethanol, and stirring until the solution isuniform and transparent; adding the antitumor drug into the aqueous solution of ethanol or water, and stirring until the solution is uniform and transparent; mixing the ethanol aqueous solution of the antitumor drug or an aqueous solution of the antitumor drug with the zein solution; performing freeze drying or spray drying on the zein nano embedded mixed solution, thereby obtaining the zein nanoembedded slow-release filler. According to the method disclosed by the invention, the zein which is high in biocompatibility, biodegradable, non-toxic and edible is utilized to achieve the effect ofperforming induced self-assembling and embedding on the antitumor drug in the ethanol solution, namely the antitumor drug can be effectively protected, and in-vivo controlled release and slow releasecan be realized.

Description

technical field [0001] The invention belongs to the technical field of chemotherapeutic drugs, and in particular relates to a zein nano-embedded slow-release filler with good stability, low toxic and side effects, high bioavailability, controlled release and slow release, and simple preparation process and a preparation method thereof . Background technique [0002] Controlled drug release is to use natural or polymer compounds as drug carriers to control the release rate of drugs in the human body, so that drugs can be released slowly within a certain time range and speed to achieve the purpose of treating a certain disease. In addition to being used in medicine, controlled release technology can also be developed in pesticides, fertilizers, cosmetics and food additives. [0003] Corn contains about 10% protein in dry matter, of which 50-60% is prolamin, which is a by-product of corn wet processing. Because zein lacks essential amino acids such as lysine and tryptophan, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K45/00A61K47/42A61P35/00
Inventor 孙洪亮仇乐杜鑫石绮君刘爽贺婷婷张莹莹
Owner 云智前沿科技发展(深圳)有限公司
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