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Composite fungicide for peanuts to resist continuous cropping, as well as preparation method and applications thereof

A composite bacterial agent and composite bacterial technology, applied in biochemical equipment and methods, botany equipment and methods, and microbial-based methods, can solve the problems of microbial ecological balance destruction, human living environment pollution, soil hardening, etc. Achieve the effect of being beneficial to absorption, improving soil fertility and high degradation efficiency

Inactive Publication Date: 2012-11-14
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] With the widespread use of chemical fertilizers, it has brought a yield revolution to the planting industry, but the continuous use of chemical fertilizers has disrupted the ecological balance of microorganisms in the soil, soil compaction and hardening, soil-borne diseases have intensified, and what is more worrying is the human living environment. polluted
The world began to study and solve the problem of soil improvement. The earliest biological fertilizers were nitrogen-fixing bacteria in the United States and BOM bacteria in Japan, etc., and they were used in my country, but the price was relatively expensive, the function was single, and the promotion was limited; most manufacturers in my country produced Most of the bacterial fertilizers are single strains with single functions and insignificant effects, and the special bacterial agents for peanut anti-continuous cropping are rarely produced and used in my country.

Method used

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  • Composite fungicide for peanuts to resist continuous cropping, as well as preparation method and applications thereof
  • Composite fungicide for peanuts to resist continuous cropping, as well as preparation method and applications thereof
  • Composite fungicide for peanuts to resist continuous cropping, as well as preparation method and applications thereof

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Embodiment 1

[0031] 1. Source of bacteria

[0032] The soil was collected from the peanut field where peanuts have been planted continuously for many years in Donglongwanzhuang, Guhe Town, Laixi City. The soils were soils of continuous peanut cropping for 1 year, 2 years, 4 years, and 7 years. The soil type is fluvo-fluvo soil, and the soil texture type is sandy soil , the peanut variety is Huayu No. 16. Brush the soil sample from the peanut root into a ziplock bag with a brush, and collect the soil within 2mm around the root system. The total soil weight is about 500g, and it will be stored in cold storage for the screening of microorganisms and the isolation and identification of allelochemicals.

[0033] 2. Strain screening method

[0034] Nutrient agar medium (NA medium): peptone 5.0g; beef extract 3.0g; glucose 2.5g; yeast powder 1.0g; agar powder 16.0-18.0g; distilled water 1000ml; pH7.0-7.2. Prepare NA liquid medium separately.

[0035] Carbon-free basal medium (CM medium): K 2 ...

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Abstract

The invention provides a composite fungicide for peanuts to resist continuous cropping, as well as a preparation method and applications of the composite fungicide, and specifically provides a composite fungicide for micrococcus lylae O6, leminorella richardii L2, ardisia japonica phyllobacterium C3 and the peanuts to resist continuous cropping. According to the composite fungicide provided by the invention, the allelochemical degrading efficiency is high; and strains existing in natural soils are taken as main materials, the degraded product can be taken as a carbon source by bacteria to be converted into a harmless subject, the carbon source in the soil is increased, the roots of crops can absorb the degraded subjects conveniently, and the environment can not be polluted. According to the composite fungicide, three strains are cultured continuously, the genic mutation can not occur, the degrading properties can be inherited stably; meanwhile, the quantity of beneficial bacteria in the soil can be increased, so that the soil fertility is increased; and furthermore, the preparation method of the composite fungicide provided by the invention is simple, the extraction process is simple, toxic substances can not be added in the preparation process, and the use is simple.

Description

technical field [0001] The invention relates to a composite bacterial agent in the biological field, in particular to a peanut anti-continuous cropping composite bacterial agent and its preparation method and application. Background technique [0002] As we all know, peanut is one of the important oil crops. Shandong Province is one of the most important peanut production areas and export bases in China. The main planting areas are in Jiaodong Peninsula, southern Shandong and central and western Shandong. The perennial planting area is generally above 85hm2, and the total output is over 350 tons, accounting for 20% and 28% of the national total respectively. 70%, the sown area, total output, per unit yield and export earnings all rank first in the country. In recent years, Shandong Province has a large peanut planting area and concentrated production areas, but there is a relatively large continuous cropping area every year, about 140,000 hm2. The continuous cropping area o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C09K17/00C12R1/265C12R1/01C09K101/00
Inventor 崔德杰袁云云咸洪泉宋杰李旭霖
Owner QINGDAO AGRI UNIV
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