Composite fungicide for peanuts to resist continuous cropping, as well as preparation method and applications thereof
A composite bacterial agent and composite bacterial technology, applied in biochemical equipment and methods, botany equipment and methods, and microbial-based methods, can solve the problems of microbial ecological balance destruction, human living environment pollution, soil hardening, etc. Achieve the effect of being beneficial to absorption, improving soil fertility and high degradation efficiency
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[0031] 1. Source of bacteria
[0032] The soil was collected from the peanut field where peanuts have been planted continuously for many years in Donglongwanzhuang, Guhe Town, Laixi City. The soils were soils of continuous peanut cropping for 1 year, 2 years, 4 years, and 7 years. The soil type is fluvo-fluvo soil, and the soil texture type is sandy soil , the peanut variety is Huayu No. 16. Brush the soil sample from the peanut root into a ziplock bag with a brush, and collect the soil within 2mm around the root system. The total soil weight is about 500g, and it will be stored in cold storage for the screening of microorganisms and the isolation and identification of allelochemicals.
[0033] 2. Strain screening method
[0034] Nutrient agar medium (NA medium): peptone 5.0g; beef extract 3.0g; glucose 2.5g; yeast powder 1.0g; agar powder 16.0-18.0g; distilled water 1000ml; pH7.0-7.2. Prepare NA liquid medium separately.
[0035] Carbon-free basal medium (CM medium): K 2 ...
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