Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip

A technology of microfluidic chips and detection chips, which is applied in the direction of chemical instruments and methods, measuring devices, and laboratory containers, can solve problems such as no substantial breakthroughs, achieve good application prospects, simplify pretreatment, The effect of shortening the detection time

Inactive Publication Date: 2012-11-21
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the centrifugal microfluidic chip analysis technology that uses a wafer-shaped chip, uses microfabrication technology to prepare microstructures and microchannels on its surface for analysis, relies on centrifu

Method used

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  • Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The microstructure and microchannel graphics of the nine-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructures and microchannels were prepared on the three-layer polymethyl methacrylate (PMMA) chip by the CNC micromachining system. The chips of each layer were cleaned with tap water and distilled water respectively, and the remaining fingerprints, oil stains and other stains on the surface of the chip were wiped with ethanol. On the two-layer Teflon film and the four-layer double-sided adhesive film, the required microstructure and microchannel are prepared by cutting plotter. Put 5 mg of soil sample or standard oil sample in the extraction cell, carefully align, bond, and press-seal the nine-layer chip to make a centrifugal microfluidic chip for detecting oil and grease. Fix the centrifugal microfluidic chip on the rotating platform of the centrifuge through a suction cup, and add 100 μL C from th...

Embodiment 2

[0030] The microstructure and microchannel graphics of the nine-layer chip of the centrifugal microfluidic chip were designed and drawn by computer-aided design software. The microstructures and microchannels were prepared on the three-layer polycarbonate (PC) chip by the numerical control CNC micromachining system. Finally, the chips of each layer were cleaned with tap water and distilled water, and the remaining fingerprints, oil stains and other stains on the surface of the chip were wiped with ethanol. On the two-layer Teflon film and the four-layer mechanoadhesive film, the required microstructure and microchannel were prepared by cutting plotter. Put 5mL water sample or standard oil sample in the extraction pool, carefully align, bond, and press-seal the nine-layer chip to make a centrifugal microfluidic chip for detecting oil and grease. Fix the centrifugal microfluidic chip on the rotating platform of the centrifuge through the card slot, and add 100 μL C from the samp...

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Abstract

The invention relates to a centrifugal micro-fluidic chip for detecting oil and grease and a preparation method of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip is a disk type centrifugal micro-fluidic chip with a microstructure and a microchannel; the centrifugal micro-fluidic chip is formed by sealing multiple layers of chips on which micro structures and micro channels with micro levels are etched, a Teflon film used as an infrared optical view window and a dual-layer sticky film; the mixing, extracting and separating process of a sample to be measured and an extraction agent is finished by taking a centrifugal force as a driving force of sample micro fluid; and the contents of the oil and the grease in the sample on each chip are quantitatively or qualitatively detected by taking an infrared spectrometer as a detection device. According to the micro-fluidic chip and the method, a plurality of groups of samples can be simultaneously treated and detected; reagents and samples are fewer, and thus the integration, the micromation and the automation of the oil and the grease are realized; and the centrifugal micro-fluidic chip has the characteristics of economy, quickness, portability and high efficiency and provides a brand-new analysis technology for detecting the oil and the grease conveniently, quickly and quantitatively.

Description

technical field [0001] The invention relates to a microfluidic chip for detecting oil and grease and a preparation method thereof. The chip is a disc-shaped centrifugal microfluidic chip, and microstructures and microchannels are prepared on the surface by micromachining technology, and produced by rotating in a centrifuge. Driven by centrifugal force, the process of mixing, extracting and separating the sample to be tested and the extractant is realized, and the content of oil and grease in the sample on the chip is quantitatively or qualitatively detected by infrared spectrometer, which is mainly used in related fields such as environmental protection and water quality detection. Background technique [0002] Serious "leakage" accidents often occur in industrial production. The most common leaked substances are hydrocarbons, such as petroleum products. Although the leaked substances are usually not very toxic, if a large amount of leakage occurs in an inappropriate area, it...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/01G01N21/35G01N35/00
CPCG01N21/07G01N21/3577
Inventor 沙俊王晓东聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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