Cordyceps sinensis host infection strain material and method
A technology of Cordyceps sinensis host and Cordyceps sinensis fungus, which is applied to the strain material and host infection field of Cordyceps sinensis host infection, which can solve the problems of difficult separation and purification of Mortierella sinensis species, low infection rate and survival rate of larvae, and reach the infection rate and the effect of improved survival rate and low preparation cost
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Embodiment 1
[0013] Embodiment 1 The preparation method of the strain material used for the host infection of Cordyceps sinensis
[0014] Collect the grown Cordyceps sinensis, and the concrete collection method is: pry up the topsoil around the Cordyceps sinensis, can not touch the surface of the sclerotium of the sclerotium of the sclerotium of the sclerotium; peel off the clods around the sclerotium of the sclerotium, and obtain a layer of bacteria whose appearance is similar to the shape of the sclerotium of the sclerotium of the sclerotium. Cordyceps sinensis summer grass wrapped in a shell composed of a film and soil mixture; collecting the shell composed of the above-mentioned bacterial film and soil mixture to obtain a cordyceps sinensis fungus skin.
[0015] Ultrasonic melting of Cordyceps sinensis fungus skin with water to obtain a suspension of Cordyceps sinensis fungus skin, filtering the soil in the suspension to obtain the first aqueous solution; washing the filtered soil with ...
Embodiment 2
[0016] The investigation of the infection method of embodiment 2 Cordyceps sinensis host 1
[0017] The body fluid of the bat moth larvae was obtained from the live body of the bat moth larvae infected with Trichospora chinensis, and the body fluid was glued with an inoculating needle and smeared (ie, inoculated) on the plate medium under aseptic conditions. The plate medium smeared with the body fluid of live larvae with bacteria was placed in an incubator at 8-18°C for cultivation. After 3 days, observe once a day to eliminate impurities in time. After 10 to 16 days, dark gray will be formed at the inoculation site of the plate medium, and then it will turn into brownish yellow or even black Trichospora chinensis colonies. The isolated Trichospora chinensis was transferred from the flat medium plate to the medium of the row, and cultured at 18°C. The center of the inoculum was yellow-brown, the colony was hard, and there were sparse and flat fibrous bacterial filaments. Con...
Embodiment 3
[0023] The investigation of the infection method of embodiment 3 Cordyceps sinensis host 2
[0024] Collect the larvae of Hepialus xiaojinensisTu etMa, and 2400 worms were divided into 8 1m 2 In a clean sterilized enamel plate, each group of 300 worms, put some sterilized dry leaves on it to facilitate the movement and concealment of the worms, and spray some distilled water to maintain humidity. First, use sterile Polygonum bulbi, carrot, red stalk, and vegetable leaves to feed for 7 days to restore the growth of the worms. The above-mentioned 6 groups were cleaned on the surface of the worms, and 3% hydrogen peroxide was used for surface disinfection; any 4 groups were selected, and the spore suspension prepared in Example 2 was injected into the worms through the micropores on the surface of the worms under a microscope with a micro-injection gun. In the body blood cavity, as control group II; select any 4 groups, use a micro-injection gun, and inject the bacterial species...
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