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Application of catclaw buttercup root extract to preparation of anti-lung cancer medicines

A technology of cat's claw and extract, applied in the field of medicine, can solve the problems of unscaled preparation production, unclear active site, short medicinal history and the like, and achieve the effects of low toxicity, simple method and low cost

Inactive Publication Date: 2012-12-05
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The medicinal history of cat's claw is relatively short. Although it has attracted people's attention in recent years due to its good anti-tumor activity when compounded with other multi-flavored Chinese medicinal materials, there are still many problems: (1) cat's claw has a wide range of resources, However, the degree of development and utilization is not enough, and the production of preparations has not reached a large scale; (2) the active ingredients and active parts of cat's claw are not clear, so the screening of its effective parts and active components should be focused on the pharmacological effects of cat's claw, in order to provide The further development and utilization of cat's claw provides scientific basis

Method used

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  • Application of catclaw buttercup root extract to preparation of anti-lung cancer medicines
  • Application of catclaw buttercup root extract to preparation of anti-lung cancer medicines
  • Application of catclaw buttercup root extract to preparation of anti-lung cancer medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Preparation of Cat's Claw Extract

[0032] Accurately weigh 5000g of dried cat's claw, crush it into coarse particles (200 mesh), and extract 4 times by heating and refluxing with 95% ethanol aqueous solution, each time the amount of ethanol aqueous solution is 8 of the weight of cat's claw. Double the amount, extract 2h each time, filter, and recover the ethanol from the alcohol extract to obtain the extract. Add warm water to 1g crude drug / ml for the extract, add petroleum ether and extract 4 times at 50℃, each volume of petroleum ether and warm water The volume ratio of the latter extract is 1:1, and each extraction is 4 hours, and the petroleum ether is recovered from the petroleum ether extract to obtain the petroleum ether extract. The remaining aqueous layer was extracted 5 times with water-saturated n-butanol, the n-butanol extract was extracted twice with 0.1 mol / L NaOH aqueous solution, the n-butanol layer was concentrated to the extract, and dried in ...

Embodiment 2

[0033] Example 2, 3 H-TdR incorporation method to observe the effect of cat's claw extract on the proliferation of A549 and NCI-H460 cells

[0034] 1. Experimental materials

[0035] (1) RPMI1640 culture medium: 250ml / bottle, produced by Jinuo Biomedical Technology Co., Ltd., batch number 20110720.

[0036] (2) 0.25% (mass percentage) Trypsin: containing 0.02% (mass percentage) ethylenediaminetetraacetic acid (EDTA), 100ml / bottle, produced by Jinuo Biomedical Technology Co., Ltd., batch number 11090901.

[0037] (3) Super newborn calf serum (ie fetal calf serum, FCS): 200ml / bottle, produced by Hangzhou Sijiqing Bioengineering Materials Co., Ltd., batch number 080304.

[0038] (4) Tritium-thymidine ( 3 H-TdR): Produced by Shanghai Institute of Applied Physics, Chinese Academy of Sciences, concentration 1mci / ml, specificity 4ci / mM, batch number 100430, prepared with Hank's solution to 20μci / ml before use.

[0039] (5) 96-well flat-bottomed culture plate: produced by American Costar;

[0040...

Embodiment 3

[0057] Example 3. The colony formation test method observes the influence of cat's claw extract on the colony formation of A549 and NCI-H460 cells

[0058] 1. Experimental materials

[0059] (1) RPMI1640 culture medium: 250ml / bottle, produced by Jinuo Biomedical Technology Co., Ltd., batch number 20110720.

[0060] (2) 0.25% (mass percentage) Trypsin: containing 0.02% (mass percentage) ethylenediaminetetraacetic acid (EDTA), 100ml / bottle, produced by Jinuo Biomedical Technology Co., Ltd., batch number 11090901.

[0061] (3) Super newborn bovine serum: 200ml / bottle, produced by Hangzhou Sijiqing Biological Engineering Materials Co., Ltd., batch number 080304.

[0062] (4) 24-well flat-bottomed culture plate: produced by American Costar;

[0063] (5) A549 cell line (human lung adenocarcinoma cell): Provided by Cancer Institute of Zhejiang Cancer Hospital, preserved in liquid nitrogen. NCI-H460 cell line (human large cell lung cancer): Provided by the Institute of Medicine, Zhejiang Academ...

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Abstract

The invention discloses application of catclaw buttercup root extracts to preparation of anti-lung cancer medicines. The anti-lung cancer medicines comprise medicines for preventing and / or treating lung cancer. The catclaw buttercup root extracts have a good anti-lung cancer effect, growth of human lung cancer cells such as human lung adenocarcinoma cells A549 and large human lung cancer cells NCI-H460 can be effectively inhibited, and the catclaw buttercup root extracts have a low toxic effect on normal cells, is high-efficient and low-toxicity, and can be used for preparing the anti-lung cancer medicines.

Description

Technical field [0001] The invention belongs to the field of medicine, and specifically relates to an application of a cat's claw extract in preparing an anti-lung cancer medicine. Background technique [0002] Lung cancer is one of the most common malignant tumors in clinical practice. The incidence of lung cancer in my country is the most common malignant tumor in cities and the third in rural areas. Lung cancer has a high degree of malignancy, complex biological characteristics, and poor prognosis. The current treatments are mainly surgery, chemotherapy, and radiotherapy. Western medicine treatment has certain advantages in reducing tumors, while TCM treatment has a direct anti-tumor effect. It can also be combined with radiotherapy and chemotherapy to increase efficiency and reduce toxicity, enhance patients' tolerance to radiotherapy and chemotherapy, and improve the quality of life. For some patients with advanced lung cancer who cannot be operated on, they can take anti-ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61P35/00
Inventor 童晔玲杨锋戴关海任泽明
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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