Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof

A dual inactivated vaccine, a technology for chicken Newcastle disease, which is applied in the field of dual inactivated chicken Newcastle disease and H9N2 subtype avian influenza vaccine and its preparation, can solve the problems of low cross-immunization protection rate, large immune dose, low antibody level, and the like, Achieve the effect of no formaldehyde residue, high antibody level and long duration

Active Publication Date: 2012-12-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For Newcastle disease and H9N2 subtype avian influenza, there are corresponding inactivated single vaccines and double inactivated vaccines, but the

Method used

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  • Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof
  • Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof
  • Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Isolation and Identification of Example 1 H9N2 Subtype Avian Influenza Virus HN03 Strain

[0025] 1. Disease data

[0026] The disease material is aseptically collected heart blood, liver, brain and lung tissues of dead sick chickens.

[0027] 2. Tissue contacts

[0028] The disease materials were used as heart blood smears and viral tissue contact slides of various organs, and stained microscopic examination was performed, and the staining microscopic examination results of viral tissue contact slides were negative.

[0029] 3. Bacterial Culture

[0030] The above disease materials were cultured with Martin broth and sheep blood culture medium (prepared according to the method in the appendix of the current "Chinese Veterinary Pharmacopoeia"), and the results showed no colony growth, indicating that the disease materials did not contain bacteria.

[0031] 4. Treatment of disease materials

[0032] Cut and grind the above disease material in a sterile container, an...

Embodiment 2

[0059] Example 2 Preparation of Chicken Newcastle Disease and H9N2 Bird Influenza Dual Inactivated Vaccine

[0060] 1. Source of poisonous seeds for seedling production:

[0061] The virus species used to manufacture this product are chicken Newcastle disease virus La Sota strain and avian influenza virus A / chicken / He Nan / 03 / 2009 / (H9N2) strain CGMCC NO: 6258, of which avian influenza virus A / chicken / He Nan / 03 / The 2009 / (H9N2) strain is abbreviated as the H9N2 subtype avian influenza virus HN03 strain.

[0062] Chicken Newcastle Disease Virus La Sota strain (China Veterinary Drug Control Institute strain number: CVCC AV1615 strain) was purchased from China Veterinary Drug Control Institute, H9N2 subtype avian influenza virus HN03 strain was obtained from the National Veterinary Biological Products Engineering Technology Research Center according to Example 1 The method was isolated, identified by the Harbin Veterinary Research Institute, and submitted to the General Microbio...

Embodiment 3

[0100] Example 3 Double-dose Inoculation Safety Test of Chicken Newcastle Disease and H9N2 Avian Influenza Dual Inactivated Vaccine

[0101] Prepare 5 batches of chicken Newcastle disease and H9N2 avian influenza dual inactivated vaccine according to Example 2 (batch numbers: 20100301, 20100502, 20100603, 20110104, 20110305 ） , before inactivation, the antigen content of Newcastle disease virus La Sota strain and avian influenza (H9 subtype) HN03 strain were both 10 8.0 EID 50 / 0.3ml / feather portion.

[0102] The research objects were 7-day-old SPF chickens (purchased from Beijing Merial Weitong Experimental Animal Co., Ltd.) and 7-day-old Sanhuang broiler chickens (purchased from Nanjing Shifosi Chicken Farm). After each batch of chickens was bought back, they were adapted for a few days to check the general condition and clinical disease status of the chickens. Weak chickens were eliminated and healthy chickens were selected to enter the test. Five batches of double-dos...

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Abstract

The invention provides a newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and a preparation method of the bivalent inactivated vaccine, which relate to the field of biological engineering. The bivalent inactivated vaccine comprises a newcastle disease virus LaSota strain and an avian influenza virus A/chicken/HeNan/03/2009/(H9N2) strain. The preparation method of the vaccine comprises the steps of preparing, concentrating and deactivating virus liquid, performing water phase preparation and oil phase preparation, and emulsify, thereby obtaining the bivalent inactivated vaccine. The newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine has good immunogenicity, creates a high antibody level, and can withstand not only the attack of homologous viruses, but also the attack of JX02 and SH01 of H9N2 subtype avian influenza viruses, which are prevalent since 2011; the bivalent inactivated vaccine can create high level antibodies and has a long persistent period; and the preparation method of the bivalent inactivated vaccine is simple and safe.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a dual inactivated vaccine of Newcastle disease and H9N2 subtype avian influenza and a preparation method thereof. Background technique [0002] Newcastle disease, also known as Asian chicken plague or pseudo-fowl plague, has high morbidity and mortality, and is a major infectious disease that endangers the poultry industry. OIE lists it as a class A disease. Newcastle disease (ND) is an acute, febrile, septic and highly contagious infectious disease of poultry caused by Newcastle disease virus (NDV), characterized by high fever, dyspnea, diarrhea, and nervous disorders. , mucosal and serosal hemorrhages. The disease has a high mortality rate and is seriously harmful to the chicken industry. It was first discovered in Indonesia in 1926, and soon it was discovered in Newtown, England, and there are popular records in all countries in the world. [0003] Avian influenza virus (AIV)...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P31/16A61K39/145A61K39/17
Inventor 于漾揭鸿英朱亚露何家惠邓碧华张小飞艾玉春刘志凌顾巍巍
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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