Multi-element composite microorganism bacterial manure and preparation method thereof

Abstract

The invention discloses a multi-element composite microorganism bacterial manure and a preparation method thereof. The method comprises the following steps of: culturing four microorganism bacterial strains for 24h in respective liquid culture medium; mixing the cultured bacterial culture according to volume percentage ratios after the quantities of viable bacteria reach 10<9>: 30 percent of bacillus megaterium (CGMCC No.4296), 30 percent of azotobacter beijerinckii (DSM No.373), 20 percent of paenibacillus polymyxa (CGMCC No.3044) and 20 percent of paracoccus sphaerophysae (ACCC 05413T); and respectively adding the mixed liquid including 5 percent of paenibacillus polymyxa culture medium, 5 percent of bacillus megaterium Pikovskaya's culture medium, 5 percent of azotobacter beijerinckii Ashby culture medium and 5 percnet of paracoccus sphaerophysae potato dextrose agar (PDA) culture medium into the composite bacteria strains according to the total volume percentage ratios of the mixed liquid, wherein the viable count of the mixed bacteria is more than or equal to 2*10<9>, the proper temperature for production is 26-28 DEG C, and a pH value is 7.0-7.2. By using the multi-element composite microorganism bacterial manure, some available effective nitrogen, phosphorus and indoleacetic acid (IAA) are supplied to plants, and the quantity of beneficial microorganism in roots, rhizosphere and root surfaces of the plants also can be increased, so that the aims of effective growth promotion and disease prevention are fulfilled.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a multi-component compound microbial bacterial fertilizer and a preparation method thereof. Background technique

[0002] Food production is a vital event in human existence, and the food crisis is one of the five major crises that human society is facing. Therefore, how to solve this imminent event is particularly important. In order to meet the nutrient elements such as nitrogen and phosphorus required for the survival and production of crops, industrial fertilizers have always been considered as the main way to achieve this goal. However, as the use of chemical fertilizers continues to increase, its negative impacts are becoming more and more obvious, such as high cost, high consumption of non-renewable energy, air pollution, soil and water quality, food safety hazards such as agriculture and animal husbandry, and damage to soil structure and microbial zones. Departm...

Examples

Embodiment 1

[0023] The specific preparation method of bacterial fertilizer:

[0024] The sources of 4 kinds of microbial strains involved in the present invention are as follows:

[0025] Phosphorus solubilizing bacteria: (Bacillus megaterium, CGMCC No.4296);

[0026] Azotobacter: (Azotobacter beijerinckii, DSM No.373);

[0027] IAA-producing bacteria: (Paenibacillus polymyxa, CGMCC No.3044);

[0028] Anti-bacteria: (Paracoccus sphaerophysae, ACCC 05413T);

[0029] The specific formulations of culture media for various strains are as follows:

[0030] (1) Phosphobacteria Pikovskaya's medium: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 5.0g, dH 2 O 1000mL, pH7.0;

[0031] (2) Azotobacter Ashby medium: KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, mannitol 10.0g, CaSO 4 ·7H 2 O 0.1g, dH 2 O 1000mL, pH7.0;

[0032] (3) Produce IAA culture medium: (NH 4 ) 2 SO 4 6.6g, ...

Embodiment 2

[0045] Taking tomatoes cultivated according to conventional management in the greenhouse as the test object, dilute the multivariate compound microbial fertilizer 300 times to make a compound microbial fertilizer dilution, and use the dilution as a base fertilizer for root irrigation at an amount of 25 kg of dilution per mu. Spray the leaves evenly. Spraying clear water was used as the control group, and each treatment group was repeated 4 times, and each repetition was 30m 2 , Determining the total weight of tomatoes during the test period. The test results showed that the tomato yield of the control group was 5.8kg / m 2 , the output of the test group was 7.9kg / m 2 , processing improved by 36.2%.

Embodiment 3

[0047] Select tomato seedlings at the three-leaf stage cultivated by conventional management in the greenhouse, dilute the multivariate compound microbial bacterial fertilizer by 300 times by volume to make a compound microbial bacterial fertilizer dilution, and apply 10kg of dilution per mu to the whole tomato seedling. Plants were evenly sprayed, and after the leaf surface was naturally dried for 48 hours, the spore suspension of Botrytis cinerea was sprayed and inoculated at an amount of 10kg / mu. 4 replicates per treatment, 30m per replicate 2 , and sterilized distilled water was used as the blank control (CK). After the onset of the blank control, regular observation, statistics and calculation of plant disease index and disease prevention effect. According to the percentage of lesion area to leaf area, disease grades are divided: grade 0 (no disease); grade 1 (50%); Grade 5 (leaves are completely dead).

[0048] The calculation formulas for disease index and disease pre...

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a multi-component compound microbial bacterial fertilizer and a preparation method thereof. Background technique

[0002] Food production is a vital event in human existence, and the food crisis is one of the five major crises that human society is facing. Therefore, how to solve this imminent event is particularly important. In order to meet the nutrient elements such as nitrogen and phosphorus required for the survival and production of crops, industrial fertilizers have always been considered as the main way to achieve this goal. However, as the use of chemical fertilizers continues to increase, its negative impacts are becoming more and more obvious, such as high cost, high consumption of non-renewable energy, air pollution, soil and water quality, food safety hazards such as agriculture and animal husbandry, and damage to soil structure and microbial zones. Departm...

Examples

Embodiment 1

[0023] The specific preparation method of bacterial fertilizer:

[0024] The sources of 4 kinds of microbial strains involved in the present invention are as follows:

[0025] Phosphorus solubilizing bacteria: (Bacillus megaterium, CGMCC No.4296);

[0026] Azotobacter: (Azotobacter beijerinckii, DSM No.373);

[0027] IAA-producing bacteria: (Paenibacillus polymyxa, CGMCC No.3044);

[0028] Anti-bacteria: (Paracoccus sphaerophysae, ACCC 05413T);

[0029] The specific formulations of culture media for various strains are as follows:

[0030] (1) Phosphobacteria Pikovskaya's medium: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, MgSO 4 ·7H 2 O 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 5.0g, dH 2 O 1000mL, pH7.0;

[0031] (2) Azotobacter Ashby medium: KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, mannitol 10.0g, CaSO 4 ·7H 2 O 0.1g, dH 2 O 1000mL, pH7.0;

[0032] (3) Produce IAA culture medium: (NH 4 ) 2 SO 4 6.6g, ...

Embodiment 2

[0045] Taking tomatoes cultivated according to conventional management in the greenhouse as the test object, dilute the multivariate compound microbial fertilizer 300 times to make a compound microbial fertilizer dilution, and use the dilution as a base fertilizer for root irrigation at an amount of 25 kg of dilution per mu. Spray the leaves evenly. Spraying clear water was used as the control group, and each treatment group was repeated 4 times, and each repetition was 30m 2 , Determining the total weight of tomatoes during the test period. The test results showed that the tomato yield of the control group was 5.8kg / m 2 , the output of the test group was 7.9kg / m 2 , processing improved by 36.2%.

Embodiment 3

[0047] Select tomato seedlings at the three-leaf stage cultivated by conventional management in the greenhouse, dilute the multivariate compound microbial bacterial fertilizer by 300 times by volume to make a compound microbial bacterial fertilizer dilution, and apply 10kg of dilution per mu to the whole tomato seedling. Plants were evenly sprayed, and after the leaf surface was naturally dried for 48 hours, the spore suspension of Botrytis cinerea was sprayed and inoculated at an amount of 10kg / mu. 4 replicates per treatment, 30m per replicate 2 , and sterilized distilled water was used as the blank control (CK). After the onset of the blank control, regular observation, statistics and calculation of plant disease index and disease prevention effect. According to the percentage of lesion area to leaf area, disease grades are divided: grade 0 (no disease); grade 1 (50%); Grade 5 (leaves are completely dead).

[0048] The calculation formulas for disease index and disease pre...

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