Novel acidified protein feed supplement, and preparation and application thereof

A protein feed and supplementary technology, applied in biochemical equipment and methods, applications, animal feed, etc., can solve the problems of animals not eating, polluting the environment, time-consuming and laborious, etc., to reduce diarrhea rate, improve protein content, and palatability. good effect

Active Publication Date: 2013-01-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main components of these corn citric acid residues are bacterial cells rich in protein and other nutrients. If they are discharged directly, it will not only pollute the environment, but also be a waste
If it is directly used as feed, because it contains high residual acid, the palatability is poor, and animals do not like to eat it, so it needs to be reprocessed, which is not only time-consuming and laborious, but also produces a lot of waste water

Method used

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  • Novel acidified protein feed supplement, and preparation and application thereof
  • Novel acidified protein feed supplement, and preparation and application thereof
  • Novel acidified protein feed supplement, and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The kind of song preparation of embodiment 1 bacterial classification

[0034] (1) Saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC20042, purchased from China Agricultural Microorganism Culture Collection Management Center):

[0035] Saccharomyces cerevisiae ACCC20042 original bacteria test tube - small triangular bottle seeds (maltose sugar solution 100ml, sugar content 7-8°Be) incubated at 30°C for 10h - transfer to a large triangle bottle (maltose sugar solution 600 ml, sugar content 7-8°Be ), cultured at 30°C for 10 hours——transferred to Karst jars (sugar water 13kg, sugar content 7-8°Be to expand cultivation) 30°C for 10 hours——expanded Karst jar yeast seeds in distiller’s jars (medium for each Put 200kg of water in the tank, 30kg of grain flour, and 10kg of bran) for a total incubation time of 20 hours to obtain the Saccharomyces cerevisiae seed liquid, which is the cell suspension of Saccharomyces cerevisiae ACCC20042.

[0036] (2) Endomycopsis fibuligera...

Embodiment 2

[0042] Embodiment 2 Preparation of novel acidified protein feed supplement

[0043] (1) Yeast culture preparation

[0044] The preparation method of the yeast culture: 4Kg of bran, 1Kg of corn flour, 4.5Kg of soybean meal, and 0.5Kg of urea are used as a fermentation substrate, and 5Kg of water is added, and sterilized by steaming for 35 minutes under a pressure of 0.12Mpa, and then closed after sterilization. The steam inlet valve is used to discharge the material after 35 minutes of stuffing to obtain a solid-state fermentation medium. Ventilate and cool to 33°C, respectively inoculate 500 g of Saccharomyces cerevisiae ACCC20042 seed solution prepared by the method in Example 1 (the inoculation amount is based on the dry weight of the solid-state fermentation medium, and the number of live bacteria reaches 1.5×10 7 cfu / g, the same below), 500g of yeast ACCC20015 seed solution (the number of viable bacteria reached 2×10 7 cfu / g), Lactobacillus ACCC11026 seed solution 40g (t...

Embodiment 3

[0047] Embodiment 3 Preparation of novel acidified protein feed supplement

[0048] (1) Yeast culture preparation

[0049] The preparation method of the yeast culture: the fermentation substrate is composed of 5Kg of bran, 0.9Kg of corn flour, 3.85Kg of soybean meal powder, and 0.25Kg of urea, adding 8Kg of water, and cooking for 30min at a pressure of 0.12Mpa for sterilization. Close the steam inlet valve, and discharge the material for 30 minutes to obtain a solid-state fermentation medium. Ventilate and cool to 33°C, respectively inoculate 500g of Saccharomyces cerevisiae seed solution prepared by the method in Example 1 (the number of live bacteria reached 1.5×10 based on the dry weight of the solid-state fermentation medium) 7 cfu / g, the same below), 500g of the seed liquid of the sporophytum sp. 7 cfu / g), Lactobacillus seed solution 400g (viable bacteria count reaches 3×10 6 cfu / g), the fermentation temperature was controlled at 28°C, and after co-fermentation and cul...

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Abstract

The invention discloses a novel acidified protein feed supplement which comprises citric acid mycelium, corn protein powder and a nutritive regulating agent in a mass ratio of 1: 1-2: 0.05-0.1, wherein the nutritive regulating agent is composed of calcium carbonate, lysine and a yeast culture in a mass ratio of 1: 0.5-1: 3-5. The novel acidified protein feed supplement provided by the invention not only has greatly increased protein content but also has the advantages of acid fragrance, good palatability and capacity of substantially improving gastrointestinal tract environment of livestock and poultry, preserving dominant bacterial community in intestinal tracts of animals, reducing the diarrhoea rate of piglings and improving productivity of livestock and poultry; the acidified protein feed supplement is convenient to use, can directly substitute a same amount of corn and soybean meal in a mixed feed for livestock and poultry, with a substitution upper limit of 20%, and can maintainan original nutritional level and original production performance without adjustment of a formula.

Description

(1) Technical field [0001] The invention relates to a novel acidified protein feed supplement and its preparation and application. (2) Background technology [0002] my country is a large animal husbandry and aquaculture country. With the high-speed growth of my country's aquaculture industry for many years, the shortage of feed resources, especially protein resources, will become more and more serious. According to the analysis of relevant experts from the Feed Research Institute of the Chinese Academy of Agricultural Sciences, by 2020, the gap between the supply and demand of protein feed in my country will reach 48 million tons. Therefore, only relying on existing conventional plant protein feeds (including cakes, beans and corn gluten powder, etc.) and animal protein feeds is far from being able to meet the needs of the development of compound feed production. Therefore, it is imperative to develop protein feed resources. [0003] The invention develops corn citric aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A01K67/02C12N1/20C12N1/18C12N1/16C12R1/225C12R1/865C12R1/645
Inventor 陈安国洪奇华李晓燕肖英平杨彩梅
Owner ZHEJIANG UNIV
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