Immunoturbidimetry kit for detecting cysteine proteinase inhibitor C and preparation method thereof

A cysteine ​​protease and immunoturbidimetric technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the advent of cysteine ​​protease inhibitor C detection kits, and achieve the goal of being less prone to human interference and detection Good stability and repeatability, good stability effect

Inactive Publication Date: 2013-01-16
苏州照康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, the latex-enhanced immunoturbidimetric method has been widely used in the detection of lipoprotein a, C-reactive protein, and antistreptolysin 0, and has achieved good social benefits. However, such cysteine ​​has not been seen at home and abroad so far. Protease Inhibitor C detection kit comes out

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of latex suspension: mix latex microspheres with cystatin C monoclonal antibody coated in phosphate buffer, rotate at 30-40 degrees overnight, wash with cleaning stock solution to obtain latex suspension , placed in an environment of 2 to 8 degrees; wherein, the particle size of the latex microspheres is 120nm; the pH of the phosphate buffer is 7.2, and the mass ratio is 30mmol / L; the pH of the cleaning storage solution is 7.4, and the composition of the cleaning storage solution The components and their percentages by weight are: surfactant 0.2%, preservative 0.05%, buffer 30mmol / L, surfactant Triton-X405, preservative Proclin-300, buffer glycine; latex microspheres and coated The cystatin C monoclonal antibody mix was mixed in equal volumes.

[0028] Preparation of application solution: Dissolve surfactant, preservative, sodium chloride and buffer in water to obtain application solution; wherein, surfactant 0.1%, preservative 0.05%, sodium chloride 5%, buf...

Embodiment 2

[0031] Preparation of latex suspension: mix latex microspheres with cystatin C monoclonal antibody coated in phosphate buffer, rotate at 30-40 degrees overnight, wash with cleaning stock solution to obtain latex suspension , placed in an environment of 2 to 8 degrees; wherein, the particle size of the latex microspheres is 600nm; the pH of the phosphate buffer is 9.2, and the mass ratio is 60mmol / L; the pH of the cleaning storage solution is 9.0, and the composition of the cleaning storage solution The components and their weight percentages are: surfactant 0.4%, preservative 0.2%, buffer 60mmol / L, surfactant is Triton-X100, preservative is sodium azide, buffer is trihydroxyaminomethane; latex micro Balls are mixed with cystatin C monoclonal antibody coated in equal volumes.

[0032] Preparation of application solution: Dissolve surfactant, preservative, sodium chloride and buffer in water to obtain application solution; wherein, surfactant 0.4%, preservative 0.2%, sodium chlo...

Embodiment 3

[0035] Preparation of latex suspension: mix latex microspheres with cystatin C monoclonal antibody coated in phosphate buffer, rotate at 30-40 degrees overnight, wash with cleaning stock solution to obtain latex suspension , placed in an environment of 2 to 8 degrees; wherein, the particle size of the latex microspheres is 180nm; the pH of the phosphate buffer is 8.0, and the mass ratio is 120mmol / L; the pH of the cleaning storage solution is 8.4, and the composition of the cleaning storage solution The components and their weight percentages are: surfactant 0.6%, preservative 0.4%, buffer 120mmol / L, surfactant is Tween-20, preservative is a mixture of sodium azide and Proclin-300, buffer is carbonate ; Latex microspheres and cystatin C monoclonal antibody are mixed in equal volumes.

[0036] Preparation of application solution: Dissolve surfactant, preservative, sodium chloride and buffer in water to obtain application solution; wherein, surfactant 0.6%, preservative 0.4%, so...

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Abstract

The invention relates to an immunoturbidimetry kit for detecting a cysteine proteinase inhibitor C and a preparation method thereof. An application liquid bottle, an antibody suspension liquid bottle and a standard substance bottle are placed in a box body; an application liquid is filled in the application liquid bottle and comprises the following components: 0.01-2% of surfactant, 0.01-1% of preservative, 0.1-20% of sodium chloride and 10-200 mmol / L of buffer solution; a latex suspension liquid coated with a monoclonal antibody of the cysteine proteinase inhibitor C is filled in the antibody suspension liquid bottle and comprises the following components: 0.05-1% of latex coated with the monoclonal antibody of the cysteine proteinase inhibitor C, 0.01-2% of surfactant, 0.01-1% of preservative and 10-200 mmol / L of buffer solution; and a standard substance of the cysteine proteinase inhibitor C is filled in the standard substance bottle. The purpose of large-batch quantitative detection on a biochemical instrument is achieved.

Description

technical field [0001] The invention relates to an immunoturbidimetric detection method, in particular to an immunoturbidimetric kit for detecting cysteine ​​protease inhibitor C and a preparation method thereof. Background technique [0002] Cystatin C rapid detection kit can be used for human urine, plasma and serum detection. Serum cystatin C is a relatively ideal endogenous marker reflecting glomerular filtration function. Research data have shown that cystatin C is more effective than serum creatinine (Scr). Higher sensitivity and specificity, ideally reflecting endogenous markers of glomerular filtration rate (GFR). [0003] Application in kidney transplantation: In kidney transplantation, the GFR of the transplanted kidney can be used as one of the nephrotoxicity indicators for observing acute and chronic rejection reactions and immunosuppressants, and the slowly increasing Scr often cannot reflect the transplanted kidney in a timely and accurate manner. GFR and oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 刘照关康英杰
Owner 苏州照康生物技术有限公司
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