Immunoturbidimetric kit

An immunoturbidimetric and kit technology, which is applied in the field of immunoturbidimetric kits for detecting neutrophil gelatinase-associated lipocalin, can solve problems such as the advent of carrier protein detection kits, and is suitable for clinical application , High sensitivity, not easy to be disturbed by human factors

Pending Publication Date: 2021-06-11
SUZHOU WEIDU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, the latex-enhanced immunoturbidimetric method has been widely used in the detection of cystatin C, lipoprotein a, C-reactive protein, and antistreptolysin 0, and has achieved good social benefits. Neutrophil-like gelatinase-associated lipocalin detection kit released

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This embodiment provides an immunoturbidimetric kit including a box body and reagents R1, R2 and R3 located in the box and independent of each other. Among them, the composition of reagent R1 is: Triton-X100 0.2wt%, sodium azide 0.1wt%, sodium chloride 200mmol / L, PBS buffer 50mmol / L. The composition of reagent R2 is: polystyrene latex microsphere 0.5wt%, Triton-X100 0.2wt%, sodium azide 0.1wt%, PBS buffer 100mmol / L. The composition of reagent R3 is: neutrophil gelatinase-associated lipocalin 3.0 mg / L, PBS buffer 100 mmol / L.

[0034] (1) Preparation of reagent R1:

[0035] Dissolve Triton-X100, sodium azide, sodium chloride and PBS buffer in water according to the ratio to obtain reagent R1.

[0036] (2) Preparation of reagent R2:

[0037] According to the volume ratio of 1:1, mix polystyrene latex microspheres with an average diameter of 200nm and coated neutrophil gelatinase-associated lipocalin monoclonal antibody in PBS buffer, at 30-40°C Rotate the reaction over...

Embodiment 2

[0041]This embodiment provides an immunoturbidimetric kit including a box body and reagents R1, R2 and R3 located in the box and independent of each other. Among them, the composition of reagent R1 is: Triton-X405 0.2wt%, Proclin-300 0.1wt%, sodium chloride 200mmol / L, glycine buffer 50mmol / L. The composition of reagent R2 is: polystyrene latex microspheres 0.5wt%, Triton-X405 0.2wt%, Proclin-300 0.1wt%, glycine buffer 100mmol / L, trehalose 0.5g / L. The composition of reagent R3 is: neutrophil gelatinase-associated lipocalin 3.0 mg / L, glycine buffer 100 mmol / L.

[0042] (1) Preparation of reagent R1:

[0043] Dissolve Triton-X100, sodium azide, sodium chloride and PBS buffer in water according to the ratio to obtain reagent R1.

[0044] (2) Preparation of reagent R2:

[0045] According to the volume ratio of 1:1, mix polystyrene latex microspheres with an average diameter of 300nm and coated neutrophil gelatinase-associated lipocalin monoclonal antibody in glycine buffer, at 3...

Embodiment 3

[0049] This embodiment provides an immunoturbidimetric kit including a box body and reagents R1, R2 and R3 located in the box and independent of each other. Wherein, the composition of reagent R1 is: Tween-20 0.2wt%, sodium azide 0.1wt%, sodium chloride 200mmol / L, Tris-HCl buffer 50mmol / L. The composition of reagent R2 is: polystyrene latex microsphere 0.5wt%, Tween-20 0.2wt%, sodium azide 0.1wt%, Tris-HCl buffer 100mmol / L, PEG6000 1wt%. The composition of reagent R3 is: neutrophil gelatinase-associated lipocalin 3.0 mg / L, carbonate buffer 100 mmol / L.

[0050] (1) Preparation of reagent R1:

[0051] Dissolve Tween-20, sodium azide, sodium chloride and Tris-HCl buffer in water according to the ratio to obtain reagent R1.

[0052] (2) Preparation of reagent R2:

[0053] According to the volume ratio of 1:1, mix polystyrene latex microspheres with an average diameter of 200nm and coated neutrophil gelatinase-associated lipocalin monoclonal antibody in Tris-HCl buffer, 30-40 R...

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Abstract

The invention discloses an immunoturbidimetric kit which comprises a kit body, a reagent R1, a reagent R2 and a reagent R3, wherein the reagent R1, the reagent R2 and the reagent R3 are placed in the kit body; the reagent R1 is prepared from the following components: 0.01-1 weight percent of surfactant, 0.05-0.5 weight percent of preservative, 150-1000 mmol / L of sodium chloride and 5-200 mmol / L of buffer solution; the reagent R2 is prepared from the following components: 0.1-0.5 weight percent of polystyrene latex microspheres, 0.01-1 weight percent of surfactant, 0.05-0.1 weight percent of preservative and 5-200 mmol / L of buffer solution; the surface of the polystyrene latex microsphere is crosslinked with a monoclonal antibody coated with neutrophil gelatinase-associated lipocalin, and the monoclonal antibody is connected with the polystyrene latex microsphere in a covalent crosslinking or physical adsorption manner; and the reagent R3 is a buffer solution containing neutrophil gelatinase-associated lipocalin. The immunoturbidimetric kit provided by the invention realizes large-scale quantitative detection of human serum neutrophil gelatinase-associated lipocalin on a biochemical analyzer, and has the advantages of simple operation, high sensitivity, difficult interference by human factors, and good detection stability and repeatability.

Description

technical field [0001] The invention relates to the technical field of immune turbidimetry, in particular to an immune turbidimetric kit for detecting neutrophil gelatinase-associated lipocalin. Background technique [0002] Neutrophil gelatinase-associated lipocalin rapid detection ELISA kit can be used for human urine, plasma and serum detection. Acute renal failure (ARF) is a common complication of cardiac surgery, nephrotoxicity, and kidney transplantation. [0003] Neutrophil gelatinase-associated lipocalin (neutrophil gelatinase-associated lipocalin, NGAL), also known as Lipocalin-2 (lipocalin-2), is a new member of the lipocalin family, is the main cause of renal dysfunction Early biomarkers. NGAL not only exists in neutrophils, but also in specific epithelial cells. For example, in the process of ischemic and toxic renal injury, NGAL in renal tubular epithelial cells will increase significantly. Within hours, the levels of NGAL in urine and blood will increase sig...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/546
CPCG01N33/6893G01N33/577G01N33/54313G01N2333/47G01N2800/347
Inventor 刘照关
Owner SUZHOU WEIDU BIOTECH CO LTD
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