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Antioxidation active synthetic peptide and purpose thereof

A technology of antioxidant activity and peptides, applied in the direction of peptides, etc., can solve the problems of limiting the large-scale production and application of antioxidant peptides, difficult to control the quality, and high cost of the method, and achieve the effect of easy artificial synthesis, low cost, and broad application prospects

Inactive Publication Date: 2014-08-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antioxidant peptides are mostly obtained after protein enzymatic hydrolysis, separation and purification, but the cost of the method is high, the separation time is long, the quality is difficult to control, and the antioxidant activity varies, which limits the large-scale production and application of antioxidant peptides

Method used

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  • Antioxidation active synthetic peptide and purpose thereof
  • Antioxidation active synthetic peptide and purpose thereof
  • Antioxidation active synthetic peptide and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the acquisition of antioxidant activity synthetic peptide

[0031] The synthesis of peptides was provided by Beijing Zhongke Yaguang Biotechnology Co., Ltd. The peptides were prepared using an Apex 396 peptide synthesizer and synthesized by Fmoc solid-phase synthesis. According to the characteristics of the three different peptide sequences Tyr-Pro-Tyr-Trp, Tyr-Pro-Trp-Tyr, and Tyr-Pro-Phe-Trp, the amino acids are connected step by step from the C-terminus to the N-terminus. Initially, the first amino acid is attached to an insoluble support resin via an acid-sensitive linker. After the Fmoc protecting group is removed with hexahydropyridine, the second Fmoc-protected amino acid is connected, and the connection methods include preactivation or "one-pot cooking". After ligation of the target sequence, the peptide chain was eluted from the resin with TFA to obtain a crude product. The synthesized peptide was purified by high performance liquid chromatogra...

Embodiment 2

[0043] Example 2, Activity Detection of Synthetic Peptides with Antioxidative Activity

[0044] Dissolve the above polypeptides 1, 2, and 3 in 50% ethanol aqueous solution to obtain polypeptide 1 solution, polypeptide 2 solution, and polypeptide 3 solution. The concentration of the mother solution is 10 mM, and then diluted with deionized water to the required concentration below.

[0045] 1. Determination of ABTS scavenging activity of antioxidant peptides by TEAC method

[0046] Mix 7mmol / L ABTS (aqueous solution) with potassium persulfate at a final concentration of 2.45mmol / L to produce ABTS + , the mixture was placed at room temperature (25°C) in the dark for 16 hours to obtain ABTS + • Solutions. ABTS + ·The solution was diluted with absolute ethanol until the absorbance value at 734nm was 0.7±0.02. Take 4ml of this solution and mix it with 40μl 0.5mM polypeptide solution to be tested, measure the absorbance at 734nm at 30°C, replace the sample with distilled water a...

Embodiment 3

[0067] Embodiment 3, the application of antioxidant peptide

[0068] 1. Antioxidant peptides are added as antioxidant ingredients to cosmetic special nutrition cream

[0069] Special effect nutrition cream (mass fraction%)

[0070]

[0071] The above-mentioned antioxidant peptide can be any one of the 3 antioxidant peptides obtained in Example 1.

[0072] Results Antioxidant peptides can be quickly absorbed by the skin due to their small molecular weight, which can delay skin aging.

[0073] 2. Antioxidant peptides are added to the mask as an antioxidant component

[0074] Cleansing mask formula (mass fraction%):

[0075]

[0076] the rest is water

[0077] The above-mentioned antioxidant peptide can be any one of the 3 antioxidant peptides obtained in Example 1.

[0078] Results Antioxidant peptides had the functions of scavenging free radicals and preventing stains.

[0079] 3. Antioxidant peptides are added to cat food as animal nutritional supplements

[0080]...

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Abstract

The invention discloses an antioxidation active synthetic peptide and a purpose thereof. a polypeptide provided by the invention is any one of the following polypeptides 1) to 3): 1) a polypeptide shown by a sequence I in a sequence table; 2) a polypeptide shown by a sequence 2 in the sequence table; and 3) a polypeptide shown by a sequence 3 in the sequence table. According to the invention, it is proved by experiments that three polypeptides with antioxidation activity are synthesized; as the antioxidation peptide has the advantages of small molecular weight, high activity, easiness in artificial synthesis, low cost and so on, the antioxidation peptide can be widely applied to large-scale production in industries, such as foods, cosmetics, medicines and the like; and the antioxidation active synthetic peptide disclosed by the invention can be specifically used as an antioxidant in aspects, such as cosmetics, food healthcare products, animal feed additives, medicines and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a synthetic peptide with antioxidant activity and its use. Background technique [0002] Modern free radical theory points out that free radicals are harmful compounds produced by the body in oxidation reactions, which can cause oxidative damage such as lipid, protein and DNA oxidative degeneration, and cause a series of chronic diseases such as diabetes, arteriosclerosis, and cancer. In food, some nutrients will be oxidized to produce hydroperoxides, which will reduce the quality of food and even cause diseases in the body of those who eat it. Therefore, searching for antioxidants to inhibit the generation of hydroperoxides has been one of the focuses of people's attention. At present, chemically synthesized antioxidants such as BHA, BHT, and PG have been widely used in the food industry to prevent food from bad oxidation, but their application scope is limited because of their pot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/107
Inventor 李博刘珊珊李耀旺
Owner CHINA AGRI UNIV
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