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Method for preparing fish bone chondroitin sulfate

A technology for chondroitin sulfate and cartilage sulfate is applied in the field of chondroitin sulfate extraction to achieve the effects of good quality, improved comprehensive utilization rate and stable quality

Inactive Publication Date: 2013-01-30
宜昌湘宜水产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for preparing chondroitin sulfate from fish bones, which can solve the problem of utilization of fish bones in fish processing, thereby increasing the added value of leftovers in aquatic product processing and improving the quality of freshwater fish. Comprehensive utilization rate of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing chondroitin sulfate by fish bone, comprising the following steps:

[0030] 1) Pretreatment of fish bones

[0031] Boil the skull and backbone of catfish for 10 minutes, drain off the fat and meat residue, crush the fish bones, dry them, and pass through a 60-mesh sieve to obtain fish bone meal;

[0032] 2) Steam explosion treatment

[0033] Put the fishbone meal into the steam explosion reactor, and carry out steam explosion treatment under the pressure of 3.0MPa for 15min;

[0034] 3) Enzymatic hydrolysis and impurity removal

[0035] Add pure water to the steam-exploded fish bone powder according to the ratio of fish bone: water = 1:1 (w / w), and add Novozymes neutral protease (Neutrase) at a ratio of 0.03% of the weight of the fish bone. Incubate enzyme hydrolysis at 40°C and 150r / min for 1 hour, then add a decolorizing agent (activated carbon: diatomaceous earth = 1:1 (w / w)) according to 3% of the fish bone mass, raise the temperature to 95°...

Embodiment 2

[0040] A method for preparing chondroitin sulfate by fish bone, comprising the following steps:

[0041] 1) Pretreatment of fish bones

[0042] Boil the skull and backbone of catfish for 15 minutes, drain off the fat and meat residue, crush the fish bones, dry them, and pass through a 60-mesh sieve to obtain fish bone meal;

[0043] 2) Steam explosion treatment

[0044] Put the fish bone powder into the steam explosion reactor, and carry out the steam explosion treatment under the pressure of 4.0MPa for 10min;

[0045] 3) Enzymatic hydrolysis and impurity removal

[0046] Add purified water to the steam-exploded fish bone powder according to the ratio of fish bone: water = 1:3 (w / w), and add Novozymes neutral protease (Neutrase) at a ratio of 0.05% of the weight of the fish bone. Incubate enzyme hydrolysis at 60°C and 120r / min for 0.5 hours, then add decolorizing agent (activated carbon: diatomaceous earth = 1:1 (w / w)) according to 5% of the fish bone mass, raise the temper...

Embodiment 3

[0051] A method for preparing chondroitin sulfate by fish bone, comprising the following steps:

[0052] 1) Pretreatment of fish bones

[0053] Boil the skull and backbone of catfish for 12 minutes, drain off the fat and meat residue, crush the fish bones, dry them, and pass through a 60-mesh sieve to obtain fish bone meal;

[0054] 2) Steam explosion treatment

[0055] Put the fishbone meal into the steam explosion reactor, and carry out steam explosion treatment under the pressure of 3.5MPa for 12min;

[0056] 3) Enzymatic hydrolysis and impurity removal

[0057] According to the ratio of fish bone: water = 1:2 (w / w), add pure water to the steam-exploded fish bone powder, and add Novozymes neutral protease (Neutrase) at a ratio of 0.05% of the fish bone mass, Incubate enzyme hydrolysis at 50°C and 130r / min for 45min, then add decolorizing agent (activated carbon: diatomaceous earth = 1:1 (w / w)) according to 4% of the fish bone mass, raise the temperature to 97°C, and heat...

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Abstract

The invention discloses a method for preparing chondroitin sulfate from fish bone, which comprises the following steps of: pretreating fish bone, subjecting the fish bone to steam explosion treatment, carrying out the enzymatic hydrolysis of the fish bone, removing impurities, carrying out centrifugal separation, settling the solution, washing the sedimentation and drying the product. The method disclosed by the invention is simple, low in production cost, short in production period, mild in reaction conditions and high in equipment utilization rate. The average yield of the method is 8.7%, and the chondroitin sulfate content of the obtained product is 92.1% on average. The method well addresses the utilization of fish bone in fish processing, thereby improving the added value of leftovers from aquatic product processing and the comprehensive utilization rate of fresh water fish.

Description

technical field [0001] The invention relates to a preparation method of chondroitin sulfate, more specifically, to a method for extracting chondroitin sulfate by using fish bones as raw materials. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide prepared from animal cartilage tissue. It is mostly present in animal cartilage, nasal bone, throat bone, cattle, horse septum, trachea, and turtle cartilage. It is an acidic mucopolysaccharide. A polysaccharide composed of repeating disaccharide units connected by D-glucuronic acid and N-acetylgalactosamine with β-1,4-glycosidic bonds, and at the C-4 position or C of N-acetylgalactosamine Sulfation occurs on the -6 hydroxyl group. Mainly used in arthritis, eye drops, etc. At present, the raw materials for the production of chondroitin sulfate basically come from the bones of terrestrial animals such as pigs and cattle. However, due to the deterioration of the living environment of terrestrial anima...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 李作卫
Owner 宜昌湘宜水产品有限公司
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