Method for preparing fish bone chondroitin sulfate

A technology for chondroitin sulfate and cartilage sulfate is applied in the field of chondroitin sulfate extraction to achieve the effects of good quality, improved comprehensive utilization rate and stable quality

Inactive Publication Date: 2013-01-30
宜昌湘宜水产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for preparing chondroitin sulfate from fish bones, which can solve the problem of utilization of f

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0028] Example 1:

[0029] A method for preparing chondroitin sulfate from fish bones includes the following steps:

[0030] 1) Pretreatment of fish bone

[0031] Boil the skull and spine of catfish for 10 minutes, drain the fat and meat residue, crush and dry the fish bones, and sieve through 60 mesh to obtain fish bone meal;

[0032] 2) Steam explosion treatment

[0033] Put the fish bone meal into the steam explosion reactor, and perform the steam explosion treatment at a pressure of 3.0 MPa for 15 minutes;

[0034] 3) Enzymatic hydrolysis and impurity removal

[0035] According to the ratio of fish bone: water = 1:1 (w / w), pure water is added to the steam-exploded fish bone meal, and Neutrase is added at a ratio of 0.03% of the mass of the fish bone. Incubate enzyme hydrolysis at 40°C and 150r / min for 1 hour, then add a decolorizing agent (activated carbon: diatomaceous earth=1:1 (w / w)) according to 3% of the mass of fish bones, heat up to 95°C, and Stir at 200r / min for 20 minutes f...

Example Embodiment

[0039] Example 2:

[0040] A method for preparing chondroitin sulfate from fish bones includes the following steps:

[0041] 1) Pretreatment of fish bone

[0042] Boil the skull and spine of catfish for 15 minutes, drain the fat and meat residue, crush and dry the fish bones, and sieve through 60 mesh to obtain fish bone meal;

[0043] 2) Steam explosion treatment

[0044] Put the fish bone meal into a steam explosion reactor, and perform steam explosion treatment at a pressure of 4.0 MPa for 10 minutes;

[0045] 3) Enzymatic hydrolysis and impurity removal

[0046] According to the ratio of fish bone: water = 1:3 (w / w), pure water is added to the steam-exploded fish bone meal, and Neutrase is added at the ratio of 0.05% of the fish bone mass. Incubate at 60°C and 120r / min for enzymatic hydrolysis for 0.5 hours, then add a decolorizing agent (activated carbon: diatomaceous earth=1:1 (w / w)) based on 5% of the mass of fish bones, heat up to 98°C, and Stir at 180r / min for 15 minutes for en...

Example Embodiment

[0050] Example 3:

[0051] A method for preparing chondroitin sulfate from fish bones includes the following steps:

[0052] 1) Pretreatment of fish bone

[0053] Boil the skull and spine of catfish for 12 minutes, drain the fat and meat residue, crush and dry the fish bones, and sieve through 60 mesh to obtain fish bone meal;

[0054] 2) Steam explosion treatment

[0055] Put the fish bone meal into the steam explosion reactor, and perform the steam explosion treatment for 12 min at a pressure of 3.5 MPa;

[0056] 3) Enzymatic hydrolysis and impurity removal

[0057] According to the ratio of fish bone: water = 1:2 (w / w), pure water is added to the steam-exploded fish bone meal, and Neutrase is added in the proportion of 0.05% of the fish bone mass. Incubate enzyme hydrolysis at 50℃, 130r / min for 45min, then add decolorant (activated carbon: diatomaceous earth=1:1 (w / w)) according to 4% of the mass of fish bone, heat up to 97℃, and use 180r Stir for 18 minutes for enzyme inactivation a...

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Abstract

The invention discloses a method for preparing chondroitin sulfate from fish bone, which comprises the following steps of: pretreating fish bone, subjecting the fish bone to steam explosion treatment, carrying out the enzymatic hydrolysis of the fish bone, removing impurities, carrying out centrifugal separation, settling the solution, washing the sedimentation and drying the product. The method disclosed by the invention is simple, low in production cost, short in production period, mild in reaction conditions and high in equipment utilization rate. The average yield of the method is 8.7%, and the chondroitin sulfate content of the obtained product is 92.1% on average. The method well addresses the utilization of fish bone in fish processing, thereby improving the added value of leftovers from aquatic product processing and the comprehensive utilization rate of fresh water fish.

Description

technical field [0001] The invention relates to a preparation method of chondroitin sulfate, more specifically, to a method for extracting chondroitin sulfate by using fish bones as raw materials. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide prepared from animal cartilage tissue. It is mostly present in animal cartilage, nasal bone, throat bone, cattle, horse septum, trachea, and turtle cartilage. It is an acidic mucopolysaccharide. A polysaccharide composed of repeating disaccharide units connected by D-glucuronic acid and N-acetylgalactosamine with β-1,4-glycosidic bonds, and at the C-4 position or C of N-acetylgalactosamine Sulfation occurs on the -6 hydroxyl group. Mainly used in arthritis, eye drops, etc. At present, the raw materials for the production of chondroitin sulfate basically come from the bones of terrestrial animals such as pigs and cattle. However, due to the deterioration of the living environment of terrestrial anima...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 李作卫
Owner 宜昌湘宜水产品有限公司
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