Method for extracting lupeol from leaves of leptosin and new application of lupeol

A technology of flower coreopsis, extraction method, application in the field of chemistry and agriculture

Inactive Publication Date: 2013-02-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although previously, it has been obtained from Huimao Daqing (Jia Liang, Min Zhida. Chinese Herbal Medicine, 2007,38(2):161-163), Tiedaomu (Lv Taisheng, Mao Shilong, Yi Yanghua. Journal of Second Military Medical University, 2001 , 22(3):241-244), somnifera (Huang Chi, H Tunon, L Bohlin. Acta Pharmaceutica Sinica, 1995, 30(8), 621-626), fig (Xu Xike, Hu Jiang, Liu Runhui, etc. Pharmacology Services and Research, 2005,5(2):138-140), Salix salix (Yang Shumin, Liu Xikui, Qing Chen, et al. Acta Pharmaceutica Sinica, 2007,42(3):285-289), Butterbur pilaris ( Deng Guanghui, Wei Shoulian, Wei Hanxun. Rese

Method used

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  • Method for extracting lupeol from leaves of leptosin and new application of lupeol
  • Method for extracting lupeol from leaves of leptosin and new application of lupeol
  • Method for extracting lupeol from leaves of leptosin and new application of lupeol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Extraction of compound lupeol

[0018] Take 5000g Coreopsis grandiflorum leaf powder, soak and extract with 100% ethanol solution (8000mL) for 6 times, and the time is 3 days each time. Stir every 6 hours during this period. After the extracts were combined, the solvent was recovered by vacuum filtration and rotary evaporation to obtain 495 g of ethanol extraction extract; 480 g of ethanol extraction extract was added to 20 times the amount of distilled water to suspend, and an equal volume of petroleum ether was added to extract 8 times, and the solvent was recovered to obtain petroleum Ether extract extract 135g; take 50g of petroleum ether extract through silica gel column chromatography, and use 100:0, 100:1, 100:2, 100:4, 15:1, 10:1, 5:1, 3: 1. 1:1 and 0:100 volume ratio of petroleum ether + ethyl acetate mixed solvent and 50:1, 10:1, 5:1 and 0:1 volume ratio of ethyl acetate + methanol mixed solvent for gradient elution , to obtain a total of 8 fractio...

experiment example 2

[0024] Experimental Example 2 Lupeol and its herbicidal activity at each extraction stage

[0025] 1) Experimental materials

[0026] Lettuce, wheat, summer solstice grass, barnyardgrass

[0027] 2) Experimental method

[0028]Agar method: soak the recipient plant seeds in 0.2% sodium hypochlorite solution for 15 minutes, rinse them with tap water for several times and place them under running water to absorb water for about 3~5 hours, then wash them with distilled water for several times, and place them evenly in a square plate with a cover. On absorbent paper, add distilled water until the seeds do not float, and place in a fully intelligent artificial climate box (HP1000GS-B type, Wuhan Ruihua Instrument Equipment Co., Ltd.) to germinate until the radicle (or seed root) reaches 3~5mm transplanted to 0.5% agar gel mixed with extracts of each stage (prepared 100mL, and evenly distributed into 3 small beakers), the extracts of each extract should be dissolved in 0.5ml DMSO b...

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Abstract

The invention relates to the field of chemical and agriculture, in particular to a method for extracting lupeol from leaves of leptosin. The method includes taking dry powder of leaves of leptosin, extracting by the aid of ethanol and petroleum ether, performing first-gradient elution by combination of petroleum ether and ethyl acetate and combination of ethyl acetate and methanol sequentially through a silica gel column chromatography, and performing second-gradient elution to prepared fraction by combination of petroleum ether and acetone so as to obtain lupeol. The invention further provides a new application of lupeol in weeding.

Description

technical field [0001] The invention relates to the fields of chemistry and agriculture, and specifically provides a method for extracting lupeol from Coreopsis grandiflorum leaves and its application. Background technique [0002] Coreopsis grandiflora ( Coreopsis grandiflora Hogg.) also known as Cosmos genus, Coreopsis angustifolia and Coreopsis phoenix leaf, is a perennial herbaceous plant of the genus Coreopsis in the family Asteraceae, native to the Americas, introduced to my country as an ornamental plant in 1938, and is one of the exotic weeds . The grass is resistant to barrenness, pollution, and strong fecundity, so it is planted in large quantities in many areas of our country, and escapes into wild weeds, with rich resources. [0003] Studies have shown that the volatile oil of the roots, stems and leaves of the plant has inhibitory effects on Escherichia coli, Staphylococcus aureus, and Bacillus megaterium (Li Xihong, Chen Rong, Ji Fujiang. Anhui Agricultural S...

Claims

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Application Information

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IPC IPC(8): C07J63/00A01N45/00A01P13/00
Inventor 罗小勇刘杰
Owner QINGDAO AGRI UNIV
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