Method for transferring anti-borer (BT) and anti-herbicide (Bar) covalent exogenous genes into saccharum officinarum

A technology of resistance to herbicides and foreign genes, applied in botany equipment and methods, using vectors to introduce foreign genetic material, angiosperms/flowering plants, etc., can solve the problems of increasing sugarcane production input, sugarcane production loss, etc., and increase production And the effect of improving agricultural operation, reducing production input, and improving resistance

Inactive Publication Date: 2013-02-06
NANJING OYANG HI TECH AGRI
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Problems solved by technology

[0003] At present, there are more than 100 kinds of weeds that harm sugarcane. The high temperature, high humidity climate and long-term bare sugarcane fields in subtropical regions are

Method used

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Examples

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Example Embodiment

[0022] The present invention will be further described in detail below in conjunction with examples, but the implementation of the present invention is not limited thereto.

[0023] The present invention provides a method for covalently introducing foreign genes for resistance to borer (BT) and herbicide (Bar) into sugarcane, and the specific method is as follows:

[0024] (1) Select acceptor material

[0025] The acceptor materials used for the genetic transformation of sugarcane (Saccharum officinarum L.) were Xintaitang 22 and Guitang 03-1403 sugarcane plants, which were provided by the Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences in this example.

[0026] Strains and plasmids: cry1Ac gene can be directly obtained. The plant expression vector plasmid was introduced by Cambia Company in Australia, and the E.coli DH5α strain was provided by the Guangxi Sugarcane Genetic Improvement Key Open Laboratory in this example.

[0027] Medium: induction medium CM 1 M...

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Abstract

The invention relates to the research field of plant adverse resistance, and discloses a method for transferring anti-borer (BT) and anti-herbicide (Bar) covalent exogenous genes into saccharum officinarum. The method mainly includes S1, construction of a plant expression vector; S2, transfer of exogenous genes; and S3, obtaining of positive transgenic plant of the saccharum officinarum through resistance screening, polymerase chain reaction (PCR) identification and the non-toxic processing. According to the method, hereditary characters of the saccharum officinarum are improved, and anti-borer and anti-herbicide capacities of the saccharum officinarum are improved to a certain extent.

Description

technical field [0001] The invention relates to the field of plant stress resistance gene research, in particular to a method for introducing borer-resistant (BT) and herbicide-resistant (Bar) covalent exogenous genes into sugarcane. Background technique [0002] Sugarcane (Saccharum officinarum L.) is the main sugar crop in the world, and sucrose accounts for about 70% of the total sugar production. my country is one of the birthplaces of sugarcane in the world, and it is also a major producer of sugarcane sugar in the world. It currently ranks third in the world in terms of sucrose production. In addition to sugar, sugar cane is also used for paper and fuel ethanol production. Similarly, the sugarcane sugar industry occupies an important position in the national economy and is one of the most important non-grain biomass energy crops to solve the world energy crisis. In recent years, stem borers are the most common and most harmful sugarcane pests in my country's sugarcan...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
Inventor 李鸣梁朝旭方位宽何姗姗马建华
Owner NANJING OYANG HI TECH AGRI
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