Dunaliella salina culture medium, semi-continuous culture mode and method for killing protozoa in dunaliella salina solution

Abstract

The invention discloses a dunaliella salina semi-continuous culture mode and a method for eliminating the pollution of protozoa in a dunaliella salina solution, relating to the field of marine microalgae culture. In the traditional dunaliella salina culture method, an f/2 culture solution formula is used, the growth rate is low, and the yield is low. When an optimized culture medium is used for culturing dunaliella salina, the growth rate is high, the beta-carotin content is high, the cost is low, and the yield can be increased by 76%. A plastic film suspended strap dunaliella salina culture mode is used instead of the traditional open cement pit dunaliella salina culture mode; and the culture mode disclosed by the invention is easy to operate, master and control, reduces the pollution possibility, and has the advantages of flexible and diversified operation process, less laceration possibility, high safety and reliability, favorable transparency, low cost, high growth rate and high yield. When the dunaliella salina solution is treated for 12 hours by adding (NH4)2CO3 according to a dose of adding 0.6g of (NH4)2CO3 into every liter of dunaliella salina solution, the protozoa in the dunaliella salina solution, such as Oxyrrhis sp., Euplotes crassus, ciliates, amoebae and the like, can be effectively killed; and the operation is convenient, and less damage is caused to dunaliella salina cells.

Description

technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a semi-continuous culture mode of salina and a method for eliminating protozoa pollution in algae liquid. Background technique [0002] Dunaliella salina is a diflagellated unicellular green alga that can tolerate high salinity (20-300‰). Salina can accumulate a large amount of β-carotene under stress conditions, up to 14% of dry weight, which is the first of all organisms in nature. Therefore, as early as 1966, Massyuk proposed that salina can be cultivated on a large scale to produce β-carotene In addition, the glycerin contained in Salina salina can reach 40% of the dry weight, which has a potential prospect of producing glycerol; Salina salina is also a good feed for aquatic animals. my country has a long coastline and numerous inland salt lakes, and has unique natural conditions for cultivating salina to produce β-carotene. [0003] Inorganic carbon...

AI Technical Summary

Problems solved by technology

However, pollution caused by high-salt-tolerant protozoa (amoeba, artemia, ciliates, and saline phalloides) often occurs in large-area cultivation in outdoor cement pools. These organisms directly prey on salina algae or compe

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