Tartary buckwheat phenylpropanoid glycoside compound and preparation method and application thereof

A technology of buckwheat phenylpropanoid glycosides and compounds is applied in the field of preparation of such tartary buckwheat phenylpropanoside compounds, and can solve the problems such as no reports on antitumor research on chemical constituents of tartary buckwheat roots.

Inactive Publication Date: 2013-04-03
MICROSCREEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the chemical constituents of tartary buckwheat root and its anti-tumor research

Method used

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  • Tartary buckwheat phenylpropanoid glycoside compound and preparation method and application thereof
  • Tartary buckwheat phenylpropanoid glycoside compound and preparation method and application thereof
  • Tartary buckwheat phenylpropanoid glycoside compound and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Preparation of phenylpropanoid glycosides 1-7

[0014] Weigh 1000g of tartary buckwheat root, and heat extract 3 times with 80% ethanol, each time the amount of 80% ethanol is 9000g, each extraction time is 2 hours, combine the extracts and concentrate to dryness to obtain the tartary buckwheat root extract 80g, then suspended with 80g of water, degreased with dichloromethane, extracted with ethyl acetate, loaded the ethyl acetate part into silica gel column chromatography, and eluted with dichloromethane-methanol gradient (50:1, 30: 1, 15:1, 5:1, 3:1), combined to obtain three fragments (I-III). Fragment I was separated by repeated silica gel column chromatography, and dichloromethane-methanol (20:1, 10:1) was eluted to obtain two eluted fragments (I 1 , I 2 ). Fragment I 1 Compound 1 and compound 2 were obtained by loading sephadex LH-20 and eluting with 80% methanol-water. Fragment I 2 Compound 3 was obtained by sephadex LH-20 chromatography elute...

Embodiment 2

[0030] Example 2: Inhibitory effect of compounds provided by the invention on the growth of related tumor cells

[0031] (1) Sample preparation: after dissolving 0.5mg of each sample of compound 1-7 with 50ul of DMSO (Merck), the concentration is 10mg / ml, take 10ul and add PBS(-)90ul to make a solution of 1000μg / ml or a uniform suspension The solution was added to the 96-well plate as the first concentration, and the subsequent concentrations were 10-fold dilutions of the previous concentration, and the final concentrations were 100, 10, 1, 0.1, 0.01, and 0.001 μg / ml.

[0032] (2) Cell lines: A549 (human lung cancer cells), HCT116 (human colon cancer cells), ZR-75-30 (human breast cancer cells), HL-60 (human leukemia cells), all of the above cell lines were provided by the Second Military Doctor Cryopreservation and passage in the Department of Pharmacognosy, School of Pharmacy, University.

[0033] (3) Culture medium: DMEM+10-15% FBS+double antibody.

[0034] (4) Test metho...

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PUM

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Abstract

The invention discloses a tartary buckwheat phenylpropanoid glycoside compound with the structural general formula (I) shown in the specification, wherein in the formula (I), R0 and R2 represent feruloyl or p-hydroxycinnamoyl respectively; R1 and R6 represent hydrogen atom, acetyl, feruloyl or p-hydroxycinnamoyl; and R3, R4 and R5 represent hydrogen atom or acetyl. The invention also discloses a preparation method of the compound and an application of the compound in preparing an anti-tumor medicine.

Description

technical field [0001] The invention relates to a class of tartary buckwheat phenylpropanoid glycoside compounds. In addition, the invention also relates to a preparation method and medical application of the tartary buckwheat phenylpropanoid glycoside compounds. Background technique [0002] Cancer is called malignant tumor in Western medicine, and it is a disease caused by the abnormal mechanism of controlling cell growth and proliferation. It has the characteristics of unlimited proliferation and easy metastasis. According to the World Health Organization, cancer is a leading cause of death worldwide, accounting for 7.6 million deaths in 2008 (approximately 13% of all deaths). The main types of cancer were: lung cancer (1.4 million deaths), stomach cancer (740,000 deaths), liver cancer (700,000 deaths), colorectal cancer (610,000 deaths), breast cancer (460,000 deaths), all More than 70% of cancer deaths occur in low- and middle-income countries. Cancer deaths worldwide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/06C07H13/04C07H1/08A61P35/00A61P35/02A23L1/30
Inventor 郑承剑胡长玲马文辉孙慧
Owner MICROSCREEN BIOTECH SHANGHAI
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