Primer and method for predicating acute rejection occurrence risk in recipient after liver transplantation

A technology for acute rejection and liver transplantation, applied in the field of liver transplantation monitoring, can solve the problem of not further strengthening the identification of the risk of acute rejection of transplant recipients, and achieve good specific results

Inactive Publication Date: 2013-04-03
THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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Problems solved by technology

[0007] Studies have shown that the SNPs of cytokines and co-stimulatory molecules are associated with the occurrence of acute rejection after various solid organ transplants, but the findings of related studies have not further strengthened the identification of acute rejection risks of transplant recipients, and established a set of qualitative and quantitative methods. Emergency Evacuation Risk Identification Model for Recipients to Receive Rational Immunosuppressive Therapy

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  • Primer and method for predicating acute rejection occurrence risk in recipient after liver transplantation
  • Primer and method for predicating acute rejection occurrence risk in recipient after liver transplantation
  • Primer and method for predicating acute rejection occurrence risk in recipient after liver transplantation

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Embodiment Construction

[0037] 1. Crowd selection

[0038] A total of 388 patients were enrolled, including 341 males and 47 females, aged 21 to 69 years. A total of 299 patients were enrolled in the retrospective study, who underwent liver transplantation from 2006 to 2009. A total of 89 recipients were enrolled in the prospective study, who underwent liver transplantation between 2010 and 2011. The etiologies of the enrolled recipients included hepatitis B infection, hepatocellular carcinoma, severe hepatitis, and decompensated cirrhosis.

[0039] 2. Immunosuppressive regimen

[0040] The plasma concentration of tacrolimus should be controlled at 10-12 ng / mL in the first month after transplantation, 8-10 ng / mL in other months of the first year, and 5-8 ng / mL thereafter. During this period, the daily dosage of mycophenolate mofetil was 1-2g. Corticosteroids were administered with 1,000 mg of prednisolone on the day of surgery, followed by 240 mg of methylprednisolone on the first postoperative d...

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Abstract

The invention discloses a primer and a method for predicating acute rejection occurrence risk in a recipient after liver transplantation. The primer comprises a pair of PCR (Polymerase Chain Reaction) amplification primers and an extension primer for sequencing amplified segments, which are respectively shown in SEQ ID NO.13-15. The method comprises the steps of: (1) detecting genotype of a single base mutation lotus rs2127015 of B7-H3 genes of the recipient; (2) detecting whether the recipient is infected by HBV (Hepatitis B Virus); and (3) calculating acute rejection occurrence risk index A of the recipient, wherein A=-1.122+1.493*B+0.817*C. The method of the invention is simple to operate, the sensitivity in predicating the acute rejection occurrence in the recipient reaches 69.8%, and the specificity is 54.7%.

Description

technical field [0001] The invention relates to the technical field of liver transplantation monitoring, in particular to a primer and a method for predicting the risk of acute rejection of recipients after liver transplantation. Background technique [0002] Liver transplantation is one of the most effective treatments for end-stage liver disease, and recipients after liver transplantation face a serious risk of acute rejection of the graft. In recent years, thanks to the widespread application of postoperative immunosuppressants and the availability of new immunosuppressants, the average incidence of acute rejection in liver transplantation centers around the world is about 15% to 45%. [0003] Genetic differences between transplant recipients and donors are the main cause of graft rejection, and T lymphocytes from the immune system play an important role in rejection. An important criterion for the pathological judgment of acute rejection is the infiltration of a large n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 余晓波周琳谢海洋郑树森
Owner THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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