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Screening method of successive cropping obstacle resistant mutants of strawberry

A mutant and mixed solution technology, applied in the field of agricultural biology, can solve the problems of lack of systematic research, immaturity and programming of strawberry resistant mutants to fusarium wilt, and achieve the effects of short cycle, simple operation and strong repeatability.

Inactive Publication Date: 2013-04-24
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

So far, there is a lack of systematic research on the induction and selection of mutants resistant to Fusarium wilt in strawberry, and the technology is not yet mature and programmed

Method used

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  • Screening method of successive cropping obstacle resistant mutants of strawberry
  • Screening method of successive cropping obstacle resistant mutants of strawberry
  • Screening method of successive cropping obstacle resistant mutants of strawberry

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Embodiment approach

[0020] 2.1 Establishment of a callus regeneration system for isolated leaf segments

[0021] After the leaves were cut into 4×4mm leaf segments, they were inoculated with different concentrations of sucrose and maltose as the carbon source and added with different concentrations of TDZ or AgNO. 3 After 50 days of light culture (25°C, 3000LUX, 14h / d) on MS solid medium, the callus induction rate and occurrence amount of each treatment were counted. 40 leaf segments were inoculated for each concentration treatment, and repeated 4 times.

[0022] 2.2 Selection of the semi-lethal dose of the mixture

[0023] Cut out a 5mm bacterium cake from the edge of the Fusarium oxysporum colony grown on the PDA plate, and transfer it to a triangular flask containing 150 mL of Czpek's culture medium under aseptic conditions, with 5 bacterium cakes per bottle. After shaking culture (25°C, 110r / min) for 21 days, centrifuge at 4000r / min for 30min, and filter with a microporous membrane with a p...

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Abstract

The invention relates to a screening method for inducing successive cropping obstacle resistant mutants of strawberry with the mixed liquor of oxysporum toxin and autotoxin p-hydroxybenzoic acid as selection pressure and belongs to the agricultural biological technical field. The strawberry blight caused by fusarium oxysporum and the root system autotoxin p-hydroxybenzoic acid are important factors causing serious successive cropping obstacle of strawberry. The screening method employs seedling leaf sections of a strawberry group as the induction materials; and in the optimal callus induction medium (MS solid culture medium, maltose (10-30 g / L), TDZ (1-4 mg / L) and AgNO3 1mg / L) containing the mixed liquor of the toxin and p-hydroxybenzoic acid in the medial lethal concentration (10% of toxin+1.0*10-4 mol L-1), three resistant mutants high in differentiation rate, good in hereditary stability and high in firmness are selected. The screening method provides new technical way and theoretical support for realizing breakthrough and innovation of resisting continuous cropping germplasm resources under the successive cropping condition of strawberry.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for using a mixture of fusarium wilt fungus Fusarium oxysporum toxin and autotoxic substance p-hydroxybenzoic acid as selection pressure to induce and screen strawberry mutants resistant to continuous cropping obstacles. Background technique [0002] Strawberry is seriously affected by continuous cropping obstacles, and autotoxic substances and soil pathogenic microorganisms are important causes of strawberry continuous cropping obstacles. At present, the allelopathy of crops has received more and more attention, and the research on the autotoxicity of root allelochemicals has become a hot spot for scholars at home and abroad to explore the root causes of continuous cropping obstacles. Autotoxicity refers to the harmful effects of a crop or microorganism releasing secondary metabolites into the environment through natural volatilization, rain and fog...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 甄文超刘雪静齐永志马燕会代丽
Owner HEBEI AGRICULTURAL UNIV.
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