Prefabricated agarose gel electrophoresis sample loading buffer and use method thereof

Abstract

The invention relates to a prefabricated agarose gel electrophoresis sample loading buffer and a use method thereof, and belongs to the technical field of agarose gel electrophoresis. The buffer is prepared from a plurality of drips of dripwise concentrated loading buffer, a plastic package and a package tongue; and the plastic package is formed by folding low-density polyethylene plastic in half and then being subjected to tight adhesion, each drip of dripwise concentrated loading buffer is uniformly injected and sealed at a crease of the plastic package, the plurality of drips of dripwise concentrated loading buffer are uniformly and dispersedly sealed at the crease of the plastic package, and the edge of the plastic package is provided with the package tongue, when a necleic acid sample is sucked into a pipettor according to the calculation when the buffer is used, the pipettor is adjusted to enable the suction amount of the pipettor to be increased by the voume of one drip of buffer, then one drip of buffer is sucked in, the uniform mixing is achieved, and the sample loading is completed. With the prefabricated agarose gel electrophoresis sample loading buffer and the use method thereof, the sample loading is achieved without repeated measurement of the concentrated loading buffer, and the operation is simple, easily handled, and time-saving and labor-saving.

Description

technical field [0001] The invention relates to a prepacked agarose gel electrophoresis sample loading buffer and a use method thereof, belonging to the technical field of agarose gel electrophoresis. Background technique [0002] Agarose gel electrophoresis is a common technique in molecular biology experiments and is generally used for the detection, separation and purification of nucleic acid samples. In the sample loading step of agarose gel electrophoresis, the usual practice is: measure a certain volume of concentrated loading buffer, mix the nucleic acid sample with the concentrated loading buffer according to a certain ratio, and then use a micro-sampler to The mixture is added to the wells of the gel. Since the volumes of various liquids involved in this step are generally less than 10 μl, and the concentrated loading buffer needs to be repeatedly measured, the operation is cumbersome, time-consuming and labor-intensive. Contents of the invention [0003] The ob...

AI Technical Summary

Problems solved by technology

Since the volumes of various liquids involved in this step are generally less than 10 μl, and the concentrated loading buffer needs to be repeatedly measured, the operation is cumbersome, time-consuming and labor-intensive.

Images