Breeding method of gerbera jamesonii homozygote plant

A homozygous and plant technology, applied in the field of breeding of gerbera homozygous plants, can solve the problems of labor-intensive chromosome ploidy identification, low haploid doubling rate, poor rapid reproduction effect, etc. Omit complex procedures and overcome the complex effect of heterozygous state
CN103109736BActive Publication Date: 2014-04-23FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
Publication Date
2014-04-23

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Abstract

The invention provides a breeding method of a gerbera jamesonii homozygote plant, belonging to the field of biotechnology. The breeding method comprises the steps of: through flower bud selection, low-temperature treatment and disinfection, picking off ovules and introducing into a same culture medium for synchronously carrying out callus induction, bud differentiation and subculture, carrying out chromosome reduplication on a primarily selected haplobiont, primarily selecting a reduplicated strain for expanding propagation and rooting culture, hybridizing in field strains, and selecting again to obtain an excellent homozygote plant. According to the invention, the callus induction, bud differentiation and subculture of ovules are carried out by using one culture medium, and thus a tissue culture flow is simplified; and by adopting a method of combining indoor indirect evaluation and field direct evaluation, the step of evaluating the ploidy of chromosome is omitted, the difficulty that a genotype is not homozygous due to a chimera existing in an obtaining and reduplication process of the haplobiont is avoided, the gerbera jamesonii homozygote plant can be simply and rapidly bred and propagated, and a selfing life with excellent and stable characters is provided for the breeding of new variety.
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Description

technical field

[0001] The invention relates to a method for breeding homozygous gerbera plants, belonging to the field of biotechnology. Background technique

[0002] Gerbera jamesonil Bolus (Gerbera jamesonil Bolus), also known as gerbera, is a perennial herbaceous flower of the genus Gerbera in the family Asteraceae. It is one of the five largest fresh cut flowers in the world. It can be used for cut flower production or potted ornamental, and is widely distributed all over the world. Because of its gorgeous colors, huge flowers, beautiful flowers and its unique cultural connotation, it is favored by consumers.

[0003] At present, there is an urgent need for high-quality, high-yielding new gerbera varieties that are resistant to low temperature, low light, and pests and diseases. The heterosis between inbred lines is greater than that between varieties, and gerbera is a typical cross-pollination crop. Due to long-term asexual reproduction, it retains high heterozygosity...

Claims

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