Method for preparing feed additive by using bacillus licheniformis

A technology of Bacillus licheniformis and feed additives, which is applied in the field of preparing feed additives by using Bacillus licheniformis, can solve the problems that the product is difficult to achieve disease prevention and growth promotion effects, it is not easy to produce Bacillus preparations, and the difficulty of solid fermentation technology is large. Easy to test and control, easy to ferment, easy to expand the effect of cultivation

Active Publication Date: 2015-01-21
高唐华农生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, solid fermentation technology is difficult, and it is not easy to produce high-quality bacillus preparations
The current production process of bacillus preparations and the formulation of the fermentation medium produce preparations with low live bacteria content and a short validity period, which makes it difficult for the product to achieve ideal disease prevention and growth promotion effects

Method used

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  • Method for preparing feed additive by using bacillus licheniformis
  • Method for preparing feed additive by using bacillus licheniformis
  • Method for preparing feed additive by using bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Utilize Bacillus licheniformis to prepare feed additive, comprise the steps:

[0030] (1) Strain preparation and activation

[0031] Make a suspension of the bacterial strains of Bacillus licheniformis purchased, add sterile water to dilute to a concentration of 10 -8 Separation by coating or streaking on the solid plate medium, picking some single colonies to measure the production capacity; after repeated screening, the strains with strong spore activity and high number of viable bacteria produced by fermentation were determined Replace the original strain. The strains of Bacillus licheniformis were purchased from China Agricultural Microorganism Culture Collection and Management Center.

[0032] (2) Production of slant strains

[0033] Configure the slant medium according to the recipe requirements. The weight parts of each component of the slant medium are: beef extract 5 g, peptone 10 g, NaCl 5 g, agar powder 20 g, add water to 1000 mL; pH is control...

Embodiment 2

[0043] Example 2. Utilize Bacillus licheniformis to prepare feed additive, comprise the steps:

[0044] (1) Strain preparation and activation

[0045] Make a suspension of the bacterial strains of Bacillus licheniformis purchased, add sterile water to dilute to a concentration of 10 -8 When it was used, it was streaked and separated on the solid plate medium, and some single colonies were picked to measure the production capacity; after repeated screening, it was determined that the strain with strong spore activity and high number of viable bacteria produced by fermentation replaced the original strain .

[0046] (2) Production of slant strains

[0047] Configure the slant medium according to the recipe requirements. The weight parts of each component of the slant medium are: 5 g of beef extract, 10 g of peptone, 5 g of NaCl, 20 g of agar powder, and add water to make up to 1000 mL; the pH is controlled at 7.4;

[0048] Heat the slant culture medium to fully dissolve each...

Embodiment 3

[0058] Example 3. Utilize Bacillus licheniformis to prepare feed additive, comprise the steps:

[0059] (1) Strain preparation and activation

[0060] Make a suspension of the bacterial strains of Bacillus licheniformis purchased, add sterile water to dilute to a concentration of 10 -8 At the time, it was spread and separated on the solid plate medium, and some single colonies were picked to measure the production capacity; after repeated screening, it was determined that the strain produced by fermentation had strong spore activity and high number of viable bacteria to replace the original strain . The bacterial strain with strong spore activity and high number of viable bacteria refers to the bacterial strain selected after trial production and comparison of its yield.

[0061] (2) Production of slant strains

[0062] Configure the slant medium according to the recipe requirements, and the parts by weight of each component of the slant medium are: beef extract 5 g, pepto...

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Abstract

The invention provides a method for preparing a feed additive by using bacillus licheniformis. The method comprises the following steps of: preparing and activating a strain, preparing a strain with an inclined surface, preparing a triangular flask liquid strain, fermenting a canned solid and the like so as to obtain the feed additive. By adopting a unique culture medium and a solid fermentation technique, the strain propagation speed is accelerated, the fermentation period is shortened, the production cost is lowered, and the spore forming rate of a product is increased; a cheap medium such as bran is used as a main culture substrate, so that the production cost is effectively lowered; the production process has the unique advantages of water conservation, energy conservation, small quantity of byproducts and environment friendliness; and the production cost is low, the product is resistant to high temperature, acid, alkali and extrusion, is small in damage in the preparation process of the feed, small in adding quantity and remarkable in feeding effect, so that the method belongs to a sanitary production technique and has large-scale popularization and application values.

Description

technical field [0001] The invention relates to the field of feed additives for feeding, in particular to a method for preparing feed additives by using bacillus licheniformis. Background technique [0002] Farmers often add a large amount of antibiotics to the feed in order to prevent diseases when raising poultry and livestock. As a result, the abuse of antibiotics is inevitable, resulting in drug residues in animals, which eventually accumulate in human bodies through the food chain, posing a serious threat human health. Studies have shown that Bacillus licheniformis is a kind of probiotics, and the preparations made of it can play the following roles when added to feed: ① Bacillus is an aerobic beneficial bacterium, which can form spores in unfavorable environments and is resistant to high temperatures. , resistant to acid, alkali and temperature changes, can quickly revive after entering the intestinal tract; and can quickly consume limited oxygen in the environment by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16A23K1/00C12R1/10
Inventor 郝志刚罗明王奎明
Owner 高唐华农生物工程有限公司
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