Method for preparing feed additive by using bacillus licheniformis
A technology of Bacillus licheniformis and feed additives, which is applied in the field of preparing feed additives by using Bacillus licheniformis, can solve the problems that the product is difficult to achieve disease prevention and growth promotion effects, it is not easy to produce Bacillus preparations, and the difficulty of solid fermentation technology is large. Easy to test and control, easy to ferment, easy to expand the effect of cultivation
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Embodiment 1
[0029] Example 1. Utilize Bacillus licheniformis to prepare feed additive, comprise the steps:
[0030] (1) Strain preparation and activation
[0031] Make a suspension of the bacterial strains of Bacillus licheniformis purchased, add sterile water to dilute to a concentration of 10 -8 Separation by coating or streaking on the solid plate medium, picking some single colonies to measure the production capacity; after repeated screening, the strains with strong spore activity and high number of viable bacteria produced by fermentation were determined Replace the original strain. The strains of Bacillus licheniformis were purchased from China Agricultural Microorganism Culture Collection and Management Center.
[0032] (2) Production of slant strains
[0033] Configure the slant medium according to the recipe requirements. The weight parts of each component of the slant medium are: beef extract 5 g, peptone 10 g, NaCl 5 g, agar powder 20 g, add water to 1000 mL; pH is control...
Embodiment 2
[0043] Example 2. Utilize Bacillus licheniformis to prepare feed additive, comprise the steps:
[0044] (1) Strain preparation and activation
[0045] Make a suspension of the bacterial strains of Bacillus licheniformis purchased, add sterile water to dilute to a concentration of 10 -8 When it was used, it was streaked and separated on the solid plate medium, and some single colonies were picked to measure the production capacity; after repeated screening, it was determined that the strain with strong spore activity and high number of viable bacteria produced by fermentation replaced the original strain .
[0046] (2) Production of slant strains
[0047] Configure the slant medium according to the recipe requirements. The weight parts of each component of the slant medium are: 5 g of beef extract, 10 g of peptone, 5 g of NaCl, 20 g of agar powder, and add water to make up to 1000 mL; the pH is controlled at 7.4;
[0048] Heat the slant culture medium to fully dissolve each...
Embodiment 3
[0058] Example 3. Utilize Bacillus licheniformis to prepare feed additive, comprise the steps:
[0059] (1) Strain preparation and activation
[0060] Make a suspension of the bacterial strains of Bacillus licheniformis purchased, add sterile water to dilute to a concentration of 10 -8 At the time, it was spread and separated on the solid plate medium, and some single colonies were picked to measure the production capacity; after repeated screening, it was determined that the strain produced by fermentation had strong spore activity and high number of viable bacteria to replace the original strain . The bacterial strain with strong spore activity and high number of viable bacteria refers to the bacterial strain selected after trial production and comparison of its yield.
[0061] (2) Production of slant strains
[0062] Configure the slant medium according to the recipe requirements, and the parts by weight of each component of the slant medium are: beef extract 5 g, pepto...
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