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Measurement method of total saponins in health food using propolis as raw material

A health food and determination method technology, which is applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of inability to measure total saponins, high recovery rate, and good accuracy, and achieve high recovery rate and accuracy Good, simple extraction method

Active Publication Date: 2015-06-03
杭州蜂之语蜂业股份有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

This method cannot determine the total saponins in health food with propolis as raw material
[0006] In summary, there is no simple extraction method, high recovery rate, good accuracy, simple and fast method for the determination of total saponins in health food with propolis as raw material

Method used

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  • Measurement method of total saponins in health food using propolis as raw material
  • Measurement method of total saponins in health food using propolis as raw material
  • Measurement method of total saponins in health food using propolis as raw material

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Embodiment Construction

[0027] The present invention will be described in further detail below through examples, and the following examples are explanations of the present invention and the present invention is not limited to the following examples.

[0028] Example.

[0029] In this example, the determination method of total saponins in the health food with propolis as raw material includes standard curve preparation process, sample processing process, test solution passing column process, color development process and total saponin calculation process.

[0030] In the standard curve making process, draw 0ml, 10ml, 20ml, 40ml, 60ml, 80ml, 100ml and 120ml of ginsenoside Re standard solutions with a concentration of 2.0mg / mL and place them in eight evaporating dishes. After the saponin Re standard solution is dried, first add 5ml of sodium hydroxide solution with a molar concentration of 0.1M, then add 20ml of water, shake well and adjust the pH value to 6.5-7.0 with hydrochloric acid to obtain eight ...

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Abstract

The invention relates to a measurement method of total saponins in health food, and particularly relates to a measurement method of total saponins in health food using propolis as a raw material. The measurement method provided by the invention comprises a standard curve making process, a sample treatment process, a test liquid column chromatography isolation process, a color developing process and a total saponins calculating process. In the standard curve making process, a standard curve is obtained by using an absorbance value and a mass of ginsenoside as curves; in the sample treatment process, liquid to be tested is obtained by taking 0.3g of the health food using the propolis as the raw material; in the test liquid column chromatography isolation process, column chromatography isolation is carried out on the liquid to be tested by using a chromatographic column, so as to obtain an evaporation pan with the liquid to be tested which is evaporated to be dry; in the color developing process, the absorbance value of the liquid to be tested is obtained; and in the total saponins calculating process, the content of the total saponins in the health food using the propolis as the raw material can be obtained. The measurement method provided by the invention has the advantages of simple extraction method, high recovery rate, good accuracy, simplicity, convenience and rapidness.

Description

technical field [0001] The invention relates to a method for determining total saponins in health food, in particular to a method for determining total saponins in health food with propolis as raw material. Background technique [0002] Propolis is a viscous substance that bees collect from plant resin and mix with their own secretions. Propolis can nourish weakness, dissolve turbid fat, and quench thirst; external use can detoxify, reduce swelling, astringe and promote muscle growth. Propolis has been widely used as a health food raw material. [0003] The components of propolis are complex and insoluble in water. The purified propolis is brown and lumpy, which is neither convenient to take nor absorb. Therefore, it is often necessary to add auxiliary materials when processing it into health food to ensure that propolis can be dispersed in the product in a fine powder state. middle. [0004] In the health food with propolis as raw material, American ginseng, ginseng and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N1/28G01N1/34
Inventor 周萍
Owner 杭州蜂之语蜂业股份有限公司
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