Measurement method of total saponins in health food using propolis as raw material
A health food and determination method technology, which is applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of inability to measure total saponins, high recovery rate, and good accuracy, and achieve high recovery rate and accuracy Good, simple extraction method
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[0027] The present invention will be described in further detail below through examples, and the following examples are explanations of the present invention and the present invention is not limited to the following examples.
[0028] Example.
[0029] In this example, the determination method of total saponins in the health food with propolis as raw material includes standard curve preparation process, sample processing process, test solution passing column process, color development process and total saponin calculation process.
[0030] In the standard curve making process, draw 0ml, 10ml, 20ml, 40ml, 60ml, 80ml, 100ml and 120ml of ginsenoside Re standard solutions with a concentration of 2.0mg / mL and place them in eight evaporating dishes. After the saponin Re standard solution is dried, first add 5ml of sodium hydroxide solution with a molar concentration of 0.1M, then add 20ml of water, shake well and adjust the pH value to 6.5-7.0 with hydrochloric acid to obtain eight ...
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