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Measurement method of total saponins in health food using propolis as raw material

A technology of health food and determination method, applied in the preparation of test samples, measurement of color/spectral characteristics, etc., can solve the problems of high recovery rate, good accuracy, inability to measure total saponins, etc., and achieve high recovery rate and accuracy. Good, simple extraction method

Active Publication Date: 2013-05-22
杭州蜂之语蜂业股份有限公司
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Problems solved by technology

This method cannot determine the total saponins in health food with propolis as raw material
[0006] In summary, there is no simple extraction method, high recovery rate, good accuracy, simple and fast method for the determination of total saponins in health food with propolis as raw material

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  • Measurement method of total saponins in health food using propolis as raw material
  • Measurement method of total saponins in health food using propolis as raw material

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Embodiment Construction

[0027] The present invention will be further described in detail by the following examples. The following examples are for explaining the present invention and the present invention is not limited to the following examples.

[0028] Examples.

[0029] The method for measuring total saponins in health foods using propolis as a raw material in this embodiment includes a standard curve preparation process, a sample processing process, a test solution column passing process, a color development process and a total saponins calculation process.

[0030] In the standard curve preparation process, respectively suck 0ml, 10ml, 20ml, 40ml, 60ml, 80ml, 100ml and 120ml of ginsenoside Re standard solution with a concentration of 2.0mg / mL into eight evaporating dishes, and wait for the ginseng in the evaporating dishes. After the saponin Re standard solution is dried, first add 5ml of sodium hydroxide solution with a molar concentration of 0.1M, and then add 20ml of water. After shaking, adjust t...

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Abstract

The invention relates to a measurement method of total saponins in health food, and particularly relates to a measurement method of total saponins in health food using propolis as a raw material. The measurement method provided by the invention comprises a standard curve making process, a sample treatment process, a test liquid column chromatography isolation process, a color developing process and a total saponins calculating process. In the standard curve making process, a standard curve is obtained by using an absorbance value and a mass of ginsenoside as curves; in the sample treatment process, liquid to be tested is obtained by taking 0.3g of the health food using the propolis as the raw material; in the test liquid column chromatography isolation process, column chromatography isolation is carried out on the liquid to be tested by using a chromatographic column, so as to obtain an evaporation pan with the liquid to be tested which is evaporated to be dry; in the color developing process, the absorbance value of the liquid to be tested is obtained; and in the total saponins calculating process, the content of the total saponins in the health food using the propolis as the raw material can be obtained. The measurement method provided by the invention has the advantages of simple extraction method, high recovery rate, good accuracy, simplicity, convenience and rapidness.

Description

Technical field [0001] The invention relates to a method for measuring total saponins in health foods, in particular to a method for measuring total saponins in health foods using propolis as a raw material. Background technique [0002] Propolis is a sticky substance made by honeybees collecting plant resin and mixing with their own secretions. Propolis can replenish weakness, reduce turbid fat, quench thirst; topical detoxification, swelling, astringency and muscle growth. Propolis has been widely used as a raw material for health food. [0003] The components of propolis are complex and insoluble in water. The purified propolis is brown and lump-shaped, which is not convenient to take and not conducive to absorption. Therefore, it is often necessary to add auxiliary materials when processing into health food to ensure that the propolis can be dispersed in the product as a fine powder in. [0004] Health foods using propolis as raw materials can be equipped with raw materials co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28G01N1/34
Inventor 周萍
Owner 杭州蜂之语蜂业股份有限公司
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