Preparation method of three-dimensional soft bracket

A software and three-dimensional technology, applied in the field of bioengineering, can solve the problems that it is difficult to meet the needs of organ software scaffolds, and cannot prepare organ software scaffolds, so as to achieve quality assurance, reduce the chance of pollution, and avoid casualties

Active Publication Date: 2013-05-29
西安博恩生物科技有限公司
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Problems solved by technology

[0007] In order to overcome the inability to prepare soft organ scaffolds with specific internal microstructures in the prior art, and it is difficult to meet the

Method used

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  • Preparation method of three-dimensional soft bracket

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Embodiment Construction

[0022] Specific implementation examples

[0023] In this example, primary liver cells are used to prepare liver tissue scaffolds to describe the preparation method of three-dimensional soft scaffolds, which specifically includes the following steps:

[0024] Step 1. Carry out CT or MRI scanning on the replaced liver, and obtain a set of tomographic images of the organ and tissue parts with a total of 30 layers from bottom to top. i , where the size of Δh is the diameter of the nozzle hole, here we take the diameter of the nozzle hole as 0.3mm, that is, Δh=0.3mm;

[0025] Step 2. Take 1.0×10 6 Primary hepatocytes from a patient were cultured. The cell culture medium was high-glucose DMEM medium, and 10ml of fetal bovine serum, 29.2mg of glutamine and 10mg of penicillin / streptomycin were added to each 100ml of culture medium. Reach the required quantity, digest with trypsin before use, then extract the obtained hepatocytes, and finally add DMEM cell culture medium to prepare a...

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Abstract

The invention discloses a preparation method of an artificial organ soft bracket for replacing intracorporeal organs, and belongs to the technical field of bioengineering. The method comprises the steps of firstly, carrying out CT or MRI scanning on an individual organ of a patient, obtaining a group of N layers of faultage images related to organs and tissue parts from bottom to top; leading the obtained model data into a biological molding machine; then taking the organ tissue cells of the individual patient to cultivate, so as to obtain the tissue cell suspension solution with specific cell density; evenly mixing the cultivated tissue cell suspension with specific cell density and hydrogel according to a certain volume ratio; and finally printing the mixture of the tissue cell suspension solution and gel by a homemade biological molding machine, so as to finish preparation of the organ soft bracket. The soft bracket is prepared by a living tissue cell and the gel; meanwhile, the micro-structure and appearance prepared from the soft bracket are effectively controlled; and the requirements of individual difference of the patients and different parts on the organ soft bracket are met.

Description

technical field [0001] The invention relates to a preparation method of an artificial organ soft support used for organ replacement in the body. The method is a preparation method of a tissue engineering three-dimensional soft support and belongs to the technical field of bioengineering. Background technique [0002] Organ defects due to disease, aging, accidents and war etc. are commonly seen in media and clinical cases. The most effective way to solve this problem is to artificially replace the defective organs. There are two main approaches to artificially replace organisms: one is to use autologous or allogeneic organ transplantation, however, the shortage and rejection of donors have always restricted tissue and organ transplantation; the other is to use artificial organs. Traditional artificial organs are usually made of metal, ceramic or polymer materials. Due to the incompleteness of the internal structure, biological activity and degradability of this artificial o...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/16
Inventor 汪焰恩魏庆华龙水军毛海龙郭叶潘飞龙叶东东张磊李欣培李川川杨明明魏生民
Owner 西安博恩生物科技有限公司
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