Aflatoxin B1 detection kit

The technology of aflatoxin and kit is applied in the field of kits for detecting aflatoxin B1, which can solve the problems of expensive equipment, complicated operation, and low sensitivity, and achieve the effects of simple structure, convenient use, and high sensitivity

Inactive Publication Date: 2013-06-05
WUXI X RES PROD DESIGN & RES
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are multiple assay methods for aflatoxins at present, such as: thin-layer chromatography TLC (sensitivity is 5 μg / kg), high performance liquid chromatography HPLC (sensitivity is 0.02 μg / kg), immunofluorescence staining (sensitivity 0.025 μg / kg / ml), but due to the disadvantages of time-consuming, low sensitivity, expensive equipment, complicated operation and not suitable for the detection of large-scale samples, it has been phased out.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aflatoxin B1 detection kit

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0011] The sample is first processed: crush the corn sample to 20 mesh, take 5 grams of the sample and put it in a test tube, add 12.5 ml of extract (methanol: water = 7: 3). Stopper and vibrate for 3 minutes, filter, and use Xinhua No. 1 paper as the filter paper. Get 1ml filtrate and dilute with 1ml distilled water or deionized water, set aside.

[0012] Reagent balance: Take out the test box, place it for more than 15 minutes, and balance to room temperature.

[0013] Number of small wells: Pipette the required microwells and place them on the reaction plate support, add 250ul of washing solution to each well, the washing solution must not overflow, let it stand for 1 minute, shake off the washing solution, pat dry on absorbent paper, and wash twice in total. Set well No. 1 as the microplate reader zeroing well, No. 2-7 as AFB 1 Standard solution control wells, and the rest are sample wells.

[0014] Immunological reaction: Add the prepared solution and sample solution ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to view more

Abstract

The invention relates to an aflatoxin B1 detection kit, and belongs to the technical field of enzyme-linked immunosorbent assay (ELISA), wherein the aflatoxin B1 detection kit is used for detecting aflatoxin B1 (short for AFB1) content in grains, feeds and food. The aflatoxin B1 detection kit comprises a box body, a 24-cell AFB1 coated reaction plate inside the box, a reaction cover plate, 13 bottles of reagents, concave bottle positions for placing the reagents, an ice bag, a frame, an instruction manual, a packet of water absorption paper, two disposable droppers and a quality detection report. The aflatoxin B1 detection kit is characterized in that the coated reaction plate adopts a 24-well reagent plate as a solid phase carrier, and the 13 bottles of the reagents comprise 6 bottles of AFB1 standard substance solutions, enzyme labeled antigen, an enzyme labeled antigen dilution buffer solution, a concentration washing solution, a sample dilution solution, a substrate solution, a coloration solution and a termination solution, and the number of the concave bottle positions is 16. The kit has characteristics of simple structure, easy use, low price, and high sensitivity, wherein the sensitivity can be more than 0.l ng/ml.

Description

technical field [0001] A method for detecting aflatoxin B 1 The kit belongs to the technical field of enzyme-linked immunosorbent assay (ELISA), and is used for the detection of aflatoxin B in grain, feed and its food 1 (referred to as AFB 1 ) content detection. Background technique [0002] Aflatoxin (AFB 1 ) is a group of potent secondary metabolites produced by the fungi Hspergillus flavus and A. parasilicus strains, especially AFB 1 It is a strong pollutant with a wide distribution range, which can cause acute poisoning death of humans and various feed animals. It can also cause aspergitoxin 1mg / kg or more and is highly toxic. Its toxicity is 10 times that of potassium cyanide and 68 times that of arsenic. times. Fever, abdominal pain, vomiting, and loss of appetite may occur after eating foods seriously contaminated by aflatoxins. In severe cases, hepatosplenomegaly, pain in the liver area, yellow staining of skin and mucous membranes, ascites, edema of the lower l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 赵春城赵黎明王晓东单金莲钱汝文
Owner WUXI X RES PROD DESIGN & RES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap