Aflatoxin B1 detection kit

Abstract

The invention relates to an aflatoxin B1 detection kit, and belongs to the technical field of enzyme-linked immunosorbent assay (ELISA), wherein the aflatoxin B1 detection kit is used for detecting aflatoxin B1 (short for AFB1) content in grains, feeds and food. The aflatoxin B1 detection kit comprises a box body, a 24-cell AFB1 coated reaction plate inside the box, a reaction cover plate, 13 bottles of reagents, concave bottle positions for placing the reagents, an ice bag, a frame, an instruction manual, a packet of water absorption paper, two disposable droppers and a quality detection report. The aflatoxin B1 detection kit is characterized in that the coated reaction plate adopts a 24-well reagent plate as a solid phase carrier, and the 13 bottles of the reagents comprise 6 bottles of AFB1 standard substance solutions, enzyme labeled antigen, an enzyme labeled antigen dilution buffer solution, a concentration washing solution, a sample dilution solution, a substrate solution, a coloration solution and a termination solution, and the number of the concave bottle positions is 16. The kit has characteristics of simple structure, easy use, low price, and high sensitivity, wherein the sensitivity can be more than 0.l ng / ml.

Description

technical field [0001] A method for detecting aflatoxin B 1 The kit belongs to the technical field of enzyme-linked immunosorbent assay (ELISA), and is used for the detection of aflatoxin B in grain, feed and its food 1 (referred to as AFB 1 ) content detection. Background technique [0002] Aflatoxin (AFB 1 ) is a group of potent secondary metabolites produced by the fungi Hspergillus flavus and A. parasilicus strains, especially AFB 1 It is a strong pollutant with a wide distribution range, which can cause acute poisoning death of humans and various feed animals. It can also cause aspergitoxin 1mg / kg or more and is highly toxic. Its toxicity is 10 times that of potassium cyanide and 68 times that of arsenic. times. Fever, abdominal pain, vomiting, and loss of appetite may occur after eating foods seriously contaminated by aflatoxins. In severe cases, hepatosplenomegaly, pain in the liver area, yellow staining of skin and mucous membranes, ascites, edema of the lower l...

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Problems solved by technology

[0004] There are multiple assay methods for aflatoxins at present, such as: thin-layer chromatography TLC (sensitivity is 5 μg / kg), high performance liquid chromatography HPLC (sensitivity is 0.02 μg / kg), immunofluorescence staining (sensitivity 0.025 μg / kg / ml), but due to the disadvantages of time-consuming, low sensitivity, expensive equipment, complicated operation and not suitable for the detection of large-scale samples, it has been phased out.

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