Method for screening traditional Chinese medicines influencing drug resistance of escherichia coil generating ESBLs (extended spectyum beta lactamase)

Abstract

The invention relates to a method for screening traditional Chinese medicines influencing the drug resistance of escherichia coil generating ESBLs (extended spectyum beta lactamase). The method comprises the following steps of: determining the minimum inhibitory concentrations of western medicines and traditional Chinese medicines to escherichia coil strains generating ESBLs through adopting a microtest tube double broth dilution method on a 96-well cell culture plate, then adding the traditional Chinese medicines with the sub minimum inhibitory concentrations into a culture medium, and culturing and determining the minimum inhibitory concentrations of the western medicines through using the microtest tube double broth dilution method again; and subculturing in the culture medium added with the traditional Chinese medicines with the same sub minimum inhibitory concentrations and the western medicines with the same concentrations through utilizing a continuous passage method until observing that the sub minimum inhibitory concentrations of the western medicines are reduced by more than 4 times than those before the passage culture, and thus judging that the traditional Chinese medicines have multidrug-resistant reversion functions on the western medicines. The method is not limited by the culture possibility of bacteria which are effected by the traditional Chinese medicines, the bactericidal effect and multidrug-resistant reversion function of the traditional Chinese medicines are not interfered with each other, so that the screening type and scope of the traditional Chinese medicines are broadened; and the method is convenient to operate, and the screening result can be accurately and quickly determined.

Description

technical field [0001] The invention relates to a method for screening traditional Chinese medicines affecting drug resistance of ESBLs-producing Escherichia coli. Background technique [0002] Escherichia coli is a common conditional pathogen, which can cause acute sepsis, peritonitis, hepatitis, pneumonia, enteritis and other severe infections in chickens, and can also be secondary or mixed with viral diseases, causing huge economic losses to the breeding industry. loss. Among them, amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin / clavulanic acid, ampicillin / sulbactam and other β-lactam ring antibiotics are commonly used important drugs for the prevention and treatment of the disease. The scale of breeding is becoming more and more intensive, the use of drugs is becoming more and more widespread, and there are more and more drug-resistant strains. There are many reports on drug resistance of Escherichia coli. Current research has confirmed that one of the main...

AI Technical Summary

Problems solved by technology

However, using the above two methods for screening, only Chinese medicines that do not affect the growth of bacteria can be screened out, which seriously reduces the scope of screening Chinese medicines. In addition, it is difficult to judge the duration of action during screening, slow screening speed, cumbersome operations and accurate results. Sexual Difficulty Disadvantages

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