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Preparation method of gentamicin sulphate

A technology of gentamicin sulfate and gentamicin, which is applied in the field of preparation of gentamicin sulfate, can solve the problems of difficult compliance and high impurities in products, and achieves the effect of simple and easy operation of production process and reduction of impurities.

Active Publication Date: 2015-01-21
福安药业集团烟台只楚药业有限公司
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, using the existing gentamicin production formula for fermentation and production, the product has high impurities, which is difficult to meet the requirements of the 2010 edition of the "Chinese Pharmacopoeia"

Method used

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Examples

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Effect test

Embodiment 1

[0051] The preparation method of gentamicin sulfate comprises the following steps:

[0052] 1) taking gentamicin sandy soil spores and placing them on spore slant medium for spore culture, the culture temperature is 36° C., and the culture time is 10 days to obtain gentamicin spores after slant culture;

[0053] The preparation method of gentamicin sand spore is:

[0054] a, the formula of sandy soil spore culture medium is calculated by weight percentage: soluble starch 1.0%, sodium chloride (NaCl) 0.05%, potassium nitrate (KNO 3 ) 0.1%, calcium carbonate (CaCO 3 )0.1%, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.02%, bran 1.8%, magnesium sulfate (MgSO 4 ) 0.02%, agar 2.0%, and the balance is water (this embodiment is specifically soluble starch 1.0g, sodium chloride (NaCl) 0.05g, potassium nitrate (KNO 3 ) 0.1g, calcium carbonate (CaCO 3 ) 0.1g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.02g, bran 1.8g, magnesium sulfate (MgSO 4 ) 0.02g, agar 2.0g, water 94.91g...

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PUM

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Abstract

The invention discloses a preparation method of gentamicin sulphate. The use levels of a spore slant culture medium, a seed culture medium and a fermenting culture medium used in the production process are improved. The method provided by the invention has the beneficial effects that impurities in a gentamicin sulphate product can be remarkably reduced so as to meet the standard of Chinese pharmacopoeia, and the production process is simple and easy to operate. Total impurities of the gentamicin sulphate product produced before improvement is greater than 10%, and sisomicin is greater than 5%, while the total impurities of the gentamicin sulphate product produced is less than 5%, sisomicin is less than 2%, and kanamycinum (micronomicin) is less than 3%.

Description

technical field [0001] The invention relates to the field of antibiotic fermentation, in particular to a preparation method of gentamicin sulfate. Background technique [0002] Gentamicin sulfate is an aminoglycoside antibiotic, which has good antibacterial effect on various Gram-negative bacteria and Gram-positive bacteria, and has good antibacterial effect on various Enterobacteriaceae bacteria such as Escherichia coli, Klebsiella, Proteus Genus, Salmonella, Shigella, Enterobacter, Serratia and Pseudomonas aeruginosa have good antibacterial effect, Neisseria and Haemophilus influenzae are moderately sensitive to this product. It also has certain effects on Brucella, Yersinia pestis, Acinetobacter, and Campylobacter fetus. It has good antibacterial effect on about 80% of methicillin-sensitive strains of Staphylococcus (including Staphylococcus aureus and coagulase-negative staphylococcus), but most of the methicillin-resistant strains are resistant to this product. The ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/48
Inventor 王玉红葛祥斌
Owner 福安药业集团烟台只楚药业有限公司
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