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Method for screening or assisting screening of high panicle-resistant germinated wheat and special primers thereof

A technology for ear germination and wheat, applied in the field of screening or auxiliary screening of high-resistant ear germination wheat, methods and special primers, can solve the problems of difficulty in screening early-generation breeding materials, failure to identify ear germination resistance, etc., and achieve accelerated wheat breeding Process, effect of improved ear germination resistance of white-grained wheat

Inactive Publication Date: 2014-10-15
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional breeding method is generally unable to identify ear germination resistance in the field in normal years, and must be manually identified indoors by sampling, which is difficult to screen a large number of early-generation breeding materials

Method used

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  • Method for screening or assisting screening of high panicle-resistant germinated wheat and special primers thereof
  • Method for screening or assisting screening of high panicle-resistant germinated wheat and special primers thereof
  • Method for screening or assisting screening of high panicle-resistant germinated wheat and special primers thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Auxiliary screening of the main QTL locus (QPhs.caas-2BL) and the acquisition of special primer pairs for high panicle-resistant germinating wheat

[0061] 1. Identification of panicle germination resistance

[0062] The hybrid offspring F of parent CA0431 resistant to panicle germination and parent CA0306 susceptible to panicle germination 2 A total of 220 plants were planted in Beijing in 2010-2011 in the generation group, with a row length of 2m and 20 seeds / row.

[0063] From June to July 2011, F in Beijing 2 The identification of ear germination resistance of the generation population, the specific method is as follows: F 2 For the generation population, 5 ears were taken from each plant, and samples were taken at the physiological maturity stage. They were first placed in a ventilated place to dry naturally for 5 days, and then placed in a refrigerator at -20°C to maintain dormancy. After all samples were taken, they were air-dried for 2 days, and the...

Embodiment 2

[0098] Example 2. Application of the main QTL locus (QPhs.caas-2BL) and special primer pairs for panicle germination resistance in identification or auxiliary identification of high panicle germination resistance

[0099] The purpose of this experiment is to further test the following two indicators: 1. The authenticity of the existence of the main QTL locus QPhs.caas-2BL for wheat resistance to panicle germination; The specificity of the effective QTL locus QPhs.caas-2BL.

[0100] F 2:3 Groups are verified:

[0101] Will F 2:3 A generation population of 220 lines was planted in Beijing and Anyang, Henan during 2011-2012. The population adopts completely randomized block design, 2 repetitions, single-row block, row length 1m, 50 grains / row, and 2 rows of parents are planted at the end of each repetition.

[0102] 1. F 2:3 Population panicle germination resistance identification

[0103] From June to July 2012, conducted 220 F in Beijing and Anyang 2:3 Identification of ...

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Abstract

The invention discloses a method and a special primer for screening or auxiliary screening of wheat with high pre-harvest sprouting resistance. The special primer for screening or auxiliary screening of the wheat with high pre-harvest sprouting resistance consists of a primer pair 1 and a primer pair 2 as follows: the primer pair 1 consists of single-stranded DNA molecules shown in sequence 1 in a sequence table and single-stranded DNA molecules shown in sequence 2 in the sequence table, and the primer pair 2 consists of single-stranded DNA molecules shown in sequence 3 in the sequence table and single-stranded DNA molecules shown in sequence 4 in the sequence table. According to the invention, the experiment proves that the invention provides the special primer which utilizes an offspring group produced by CA0431 / CA0306 for QTL (Quantitative Trait Locus) mapping to find out a molecular marker QTL (QPhs.caas-2BL) which is closely linked with a CA0431 pre-harvest sprouting resistance gene, and screens out the special primer for auxiliary screening of the wheat with the pre-harvest sprouting resistance based on the main effect QTL. The special primer can be utilized for screening or auxiliary screening of the wheat with pre-harvest sprouting resistance so as to cultivate pre-harvest sprouting resistant varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for screening or assisting in screening high panicle-resistant germination wheat and special primers thereof. Background technique [0002] Ear germination (Pre-harvest sprouting referred to as PHS) means that wheat encounters continuous rainy weather or germinates on ears in a humid environment before harvest. Spike germination not only affects yield, but also seriously affects quality (especially processing quality), storage, and next year's sowing quality, causing different economic losses. [0003] Many countries and regions in the world have been harmed by ear germination, among which Canada and Australia are the most serious. According to Derera reports, an average of 7% of the wheat in Australia is damaged by ear germination every year. In 1984, the most serious year, the degree of ear germination damage was as high as 20%. On average, Canada loses nearly $100 milli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 陈新民苗西磊何中虎
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI