Processing method of cow urine iTRAQ test data
A technology of data and urine, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the sensitivity and accuracy are difficult to achieve the ideal effect
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[0014] According to the present invention, a method for analyzing iTRAQ data is applied to the analysis of protein iTRAQ analysis data. In the present invention, the iTRAQ analysis data of cow urine will be used as an example to illustrate the specific implementation steps of the method:
[0015] Step 1. Differential protein screening. The raw data of the screening comes from the analysis results of the proteinpilot software after iTRAQ detection of dairy cow urine. The data were grouped by experiment and screened between groups to obtain differentially expressed proteins.
[0016] Step 2: GO (Gene ontology) analysis. The differentially expressed proteins obtained in the previous step can be used as input data for GO analysis, and the differentially expressed proteins are transferred to the Gene ontology database ( www.geneontology.org) to map each node and calculate the number of genes at each node. Differential genes are classified according to biological process.
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