Reagent device for detecting mumps virus antibody and method thereof

Abstract

The invention discloses a reagent device for detecting a mumps virus antibody and a method thereof; the reagent device is long strip-shaped, comprises a substrate with eight hole sites and a handle disposed at one end of the substrate; the eight hole sites comprise a sample hole, an adjuvant hole, an enzyme conjugate hole, a substrate hole, a stopping solution hole, a diluent hole, a reaction hole, and a dilution hole, which are arranged in sequence from the end near the handle. The reagent device of the invention realizes mumps virus antibody detection with the device based on a principle of enzyme-linked immunoassay, is an independent, single-sample analytic detection method, and can be used cooperatively with corresponding specific analytical instruments; during detection, a detection reagent or a sample is injected by using a full automatic precision liquid filling device, which provides advantages of operation automation, accurate filling amount, high accuracy and precision of detection results; and the reagent device has wide application prospects.

Description

technical field [0001] The application of the present invention relates to a reagent device and a method for detecting mumps virus antibodies, which belong to the technical field of clinical immunology detection. Background technique [0002] Epidemic parotitis (mumps), referred to as mumps for short, is a common respiratory infectious disease among children and adolescents, caused by the mumps virus. The virus mainly invades the parotid gland, and various glandular tissues such as testis, ovary, pancreas, intestinal gland, thymus, and thyroid gland have the opportunity to be invaded, and can also invade almost all organs such as the nervous system, liver, kidney, and heart joints. Therefore, in addition to parotid gland swelling and pain, it can often cause symptoms such as meningoencephalitis, orchitis, pancreatitis and oophoritis. Pregnant women can infect the fetus through the placenta, resulting in fetal malformation or death, thereby increasing the incidence of miscar...

AI Technical Summary

Problems solved by technology

[0011] (1) Due to the use of closed detection, the detection steps of post-amplification electrophoresis are reduced, so the size of the amplified product cannot be monitored;

[0012] (2) Due to the limitations of the type of fluorescein and the detection light source, the application ability of the compound detection of real-time fluorescent quantitative PCR is relatively limited;

[0013] (3) At present, the cost of real-time fluorescent quantitative PCR experiment is relatively high, which also limits its wide application

[0016] (1) Use 12×8 type, 6×8 type, 8×12 type or full-plate type 96-well special microwell plate as antigen coating equipment and reaction container, which can only be divided into 12 batches and 6 batches when used , 8 batches or the whole board can be used at one time, and independent and single-person testing cannot be carried out;

[0017] (2) There are many types of reagents used in the determination, and each detection reagent must be filled with a reagent bottle, and each time a reagent is used, it is necessary to replace the suction nozzle to add to the microwells of the microwell plate respectively, Not only are there many types of reagent bottles, but also the operation of filling reagents is extremely cumbersome;

[0018] (3) There is a lack of corresponding labeling of the testing information, and the production batch number and expiration date information of the testing reagent can only be known or known by checking the label on the outer packaging box of the kit, and the known information is not controlled during the testing process, which has great potential large randomness;

[0019] (4) The detection reagent is in an open space during the detection process, which may easily cause cross-contamination between various reagents and affect the accuracy of the detection result;

[0020] (5) Manual operation is mostly used in the detection process, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur, and the accuracy and precision of the detection results are poor;

[0021] (6) The quantity configuration and use of the complete set of reagents for the test items are the number of items × 48 / 96 persons. If 10 items need to be tested, the configuration and use of the reagents must be 10 × 48 / 96 persons. If Only one sample needs to detect 10 different items, and it also needs to configure reagents for 10×48 / 96 people, which has the disadvantage of not being economical and reasonable

Images